The effects of environmental noise on in vitro immune cell function of rats

2.50
Hdl Handle:
http://hdl.handle.net/10755/150322
Type:
Presentation
Title:
The effects of environmental noise on in vitro immune cell function of rats
Abstract:
The effects of environmental noise on in vitro immune cell function of rats
Conference Sponsor:Sigma Theta Tau International
Conference Year:1991
Author:McCarthy, Donna, PhD
P.I. Institution Name:University of Wisconsin-Madison, School of Nursing
Title:Assistant Professor
It has been reported that exposure to increased environmental noise impairs wound healing in surgical patients and rats. The purpose of the present study was to determine if increased environmental noise would alter the biological function of leukocytes involved in wound healing. Male Sprague-Dawley rats were exposed to 60-85 decibels of rock radio music for 90 minutes every two hours for 24 hours. Control animals were exposed to only the usual 30-60 decibels of noises of the animal care facility. Following the 24 hour period of noise stress, whole blood was taken by cardiac puncture from both the experimental noise-exposed (n-e) and control animals. Neutrophils (PMN) and lymphocytes (LY) were separated by density, centrifugation and sedimentation. Monocytes (MO) were obtained by sterile lavage of the peritoneal cavity.



Methods: LY were incubated in the presence of concanavalin-A to induce proliferation and plasma from control and n-e animals was added in a cross-over design. Proliferation was determined as cell uptake of a radio-labeled DNA precursor (cpm). MO were incubated overnight in medium, or in medium plus endotoxin to induce interleukin-1 (IL-1) secretion. IL-1 content of the fluids was determined using the standard mouse-thymocyte assay and results are reported as c.p.m. of triplicate cultures for each supernatant. PMN were incubated in the presence of opsonized zymosan and mean nanomoles of superoxide release was determined as a colormetric change in the cell cultures measured at 9O minutes after stimulation. Data were analyzed using independent and paired t-tests of group means.



Results: Superoxide production by PMN and IL-1 secretion by MO were significantly suppressed in cells from n-e animals. LY proliferation was suppressed only if the cells were incubated in the presence of plasma from the n-e animals, suggesting the presence of an antiproliferative factor in the plasma of n-e animals. We conclude that exposure to increased environmental noise may alter biological functioning of leukocytes involved in wound healing. The data suggests that management of environmental noise may be one way nurses can intervene to promote wound healing.
Repository Posting Date:
26-Oct-2011
Date of Publication:
17-Oct-2011
Sponsors:
Sigma Theta Tau International

Full metadata record

DC FieldValue Language
dc.typePresentationen_GB
dc.titleThe effects of environmental noise on in vitro immune cell function of ratsen_GB
dc.identifier.urihttp://hdl.handle.net/10755/150322-
dc.description.abstract<table><tr><td colspan="2" class="item-title">The effects of environmental noise on in vitro immune cell function of rats</td></tr><tr class="item-sponsor"><td class="label">Conference Sponsor:</td><td class="value">Sigma Theta Tau International</td></tr><tr class="item-year"><td class="label">Conference Year:</td><td class="value">1991</td></tr><tr class="item-author"><td class="label">Author:</td><td class="value">McCarthy, Donna, PhD</td></tr><tr class="item-institute"><td class="label">P.I. Institution Name:</td><td class="value">University of Wisconsin-Madison, School of Nursing</td></tr><tr class="item-author-title"><td class="label">Title:</td><td class="value">Assistant Professor</td></tr><tr class="item-email"><td class="label">Email:</td><td class="value">domccart@facstaff.wisc.edu</td></tr><tr><td colspan="2" class="item-abstract">It has been reported that exposure to increased environmental noise impairs wound healing in surgical patients and rats. The purpose of the present study was to determine if increased environmental noise would alter the biological function of leukocytes involved in wound healing. Male Sprague-Dawley rats were exposed to 60-85 decibels of rock radio music for 90 minutes every two hours for 24 hours. Control animals were exposed to only the usual 30-60 decibels of noises of the animal care facility. Following the 24 hour period of noise stress, whole blood was taken by cardiac puncture from both the experimental noise-exposed (n-e) and control animals. Neutrophils (PMN) and lymphocytes (LY) were separated by density, centrifugation and sedimentation. Monocytes (MO) were obtained by sterile lavage of the peritoneal cavity.<br/><br/><br/><br/>Methods: LY were incubated in the presence of concanavalin-A to induce proliferation and plasma from control and n-e animals was added in a cross-over design. Proliferation was determined as cell uptake of a radio-labeled DNA precursor (cpm). MO were incubated overnight in medium, or in medium plus endotoxin to induce interleukin-1 (IL-1) secretion. IL-1 content of the fluids was determined using the standard mouse-thymocyte assay and results are reported as c.p.m. of triplicate cultures for each supernatant. PMN were incubated in the presence of opsonized zymosan and mean nanomoles of superoxide release was determined as a colormetric change in the cell cultures measured at 9O minutes after stimulation. Data were analyzed using independent and paired t-tests of group means.<br/><br/><br/><br/>Results: Superoxide production by PMN and IL-1 secretion by MO were significantly suppressed in cells from n-e animals. LY proliferation was suppressed only if the cells were incubated in the presence of plasma from the n-e animals, suggesting the presence of an antiproliferative factor in the plasma of n-e animals. We conclude that exposure to increased environmental noise may alter biological functioning of leukocytes involved in wound healing. The data suggests that management of environmental noise may be one way nurses can intervene to promote wound healing.</td></tr></table>en_GB
dc.date.available2011-10-26T10:21:40Z-
dc.date.issued2011-10-17en_GB
dc.date.accessioned2011-10-26T10:21:40Z-
dc.description.sponsorshipSigma Theta Tau Internationalen_GB
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