A Comparison of the Combination of 5-Fluorouracil and Natural Chemotherapeutic Agents, Green Tea and Thymoquinone, Delivered by Conventional Delivery on Human Colon Cancer Cells

2.50
Hdl Handle:
http://hdl.handle.net/10755/150513
Type:
Presentation
Title:
A Comparison of the Combination of 5-Fluorouracil and Natural Chemotherapeutic Agents, Green Tea and Thymoquinone, Delivered by Conventional Delivery on Human Colon Cancer Cells
Abstract:
A Comparison of the Combination of 5-Fluorouracil and Natural Chemotherapeutic Agents, Green Tea and Thymoquinone, Delivered by Conventional Delivery on Human Colon Cancer Cells
Conference Sponsor:Sigma Theta Tau International
Conference Year:2009
Author:Norwood, Anne A., PhD, RN, MSN, CS-FNP
P.I. Institution Name:University of Mississippi Medical Center School of Nursing
Title:Assistant Professor
Co-Authors:Michelle A. Tucci, PhD; Hamed A. Benghuzzi, PhD
[Evidence-based Practice Session Presentation] Colon cancer is the second most commonly occurring cancer in the world today, the second most frequently diagnosed malignancy in the United States, and the second-leading cause of cancer-related deaths in the United States for both men and women combined.  Additionally, approximately 108,070 new cases of colon cancer, as well as 49,960 deaths are expected in 2008 in North America alone with an average $8.4 billion spent per year for this type of preventable cancer (National Center for Health Statistics, 2008).  While 5-fluorouracil continues to be the chemotherapeutic gold-standard for the treatment of colon cancer, the side effects of 5-FU are numerous due to its ability to attack both healthy and cancerous cells.  However, research continues to provide positive findings in regards to antioxidants and their success in deterring cancer.  Epigallocatechin-3-gallate (EGCG), the most abundant catechin found in green tea, is a valuable scavenger of reactive oxygen species in vitro as well as in vivo.  Thymoquinone (TQ), the major active component of Nigella sativa (black seed), is also known for its powerful scavenger abilities as an inhibitor of oxidative stress and has been utilized in the Middle East for centuries because of its capability to heal many different diseases.  Therefore, the objective of this study was to investigate the effectiveness of conventional drug delivery of 5-FU in combination with TQ and EGCG on the metabolic activity and structure of the SW-626 human colon cancer cell line in culture.  Cells demonstrated significant (p< 0.05) damage when exposed to the combination of TQ/EGCG and EGCG/5-FU at 24, 48 and 72 hours. Morphological evaluation confirmed these results as well as others by exhibiting unidentifiable cell membranes at 24, 48 and 72 hours.  Results of this study support the suggestion that natural agents may offer a safe, alternative treatment for patients with colon cancer.
Repository Posting Date:
26-Oct-2011
Date of Publication:
17-Oct-2011
Sponsors:
Sigma Theta Tau International

Full metadata record

DC FieldValue Language
dc.typePresentationen_GB
dc.titleA Comparison of the Combination of 5-Fluorouracil and Natural Chemotherapeutic Agents, Green Tea and Thymoquinone, Delivered by Conventional Delivery on Human Colon Cancer Cellsen_GB
dc.identifier.urihttp://hdl.handle.net/10755/150513-
dc.description.abstract<table><tr><td colspan="2" class="item-title">A Comparison of the Combination of 5-Fluorouracil and Natural Chemotherapeutic Agents, Green Tea and Thymoquinone, Delivered by Conventional Delivery on Human Colon Cancer Cells</td></tr><tr class="item-sponsor"><td class="label">Conference Sponsor:</td><td class="value">Sigma Theta Tau International</td></tr><tr class="item-year"><td class="label">Conference Year:</td><td class="value">2009</td></tr><tr class="item-author"><td class="label">Author:</td><td class="value">Norwood, Anne A., PhD, RN, MSN, CS-FNP</td></tr><tr class="item-institute"><td class="label">P.I. Institution Name:</td><td class="value">University of Mississippi Medical Center School of Nursing</td></tr><tr class="item-author-title"><td class="label">Title:</td><td class="value">Assistant Professor</td></tr><tr class="item-email"><td class="label">Email:</td><td class="value">anorwood@son.umsmed.edu</td></tr><tr class="item-co-authors"><td class="label">Co-Authors:</td><td class="value">Michelle A. Tucci, PhD; Hamed A. Benghuzzi, PhD</td></tr><tr><td colspan="2" class="item-abstract">[Evidence-based Practice Session Presentation] Colon cancer is the second most commonly occurring cancer in the world today, the second most frequently diagnosed malignancy in the United States, and the second-leading cause of cancer-related deaths in the United States for both men and women combined. &nbsp;Additionally, approximately 108,070 new cases of colon cancer, as well as 49,960 deaths are expected in 2008 in North America alone with an average $8.4 billion spent per year for this type of preventable cancer (National Center for Health Statistics, 2008).&nbsp; While 5-fluorouracil continues to be the chemotherapeutic gold-standard for the treatment of colon cancer, the side effects of 5-FU are numerous due to its ability to attack both healthy and cancerous cells.&nbsp; However, research continues to provide positive findings in regards to antioxidants and their success in deterring cancer.&nbsp; Epigallocatechin-3-gallate (EGCG), the most abundant catechin found in green tea, is a valuable scavenger of reactive oxygen species in vitro as well as in vivo.&nbsp; Thymoquinone (TQ), the major active component of Nigella sativa (black seed), is also known for its powerful scavenger abilities as an inhibitor of oxidative stress and has been utilized in the Middle East for centuries because of its capability to heal many different diseases.&nbsp; Therefore, the objective of this study was to investigate the effectiveness of conventional drug delivery of 5-FU in combination with TQ and EGCG on the metabolic activity and structure of the SW-626 human colon cancer cell line in culture.&nbsp; Cells demonstrated significant (p&lt; 0.05) damage when exposed to the combination of TQ/EGCG and EGCG/5-FU at 24, 48 and 72 hours. Morphological evaluation confirmed these results as well as others by exhibiting unidentifiable cell membranes at 24, 48 and 72 hours.&nbsp; Results of this study support the suggestion that natural agents may offer a safe, alternative treatment for patients with colon cancer.</td></tr></table>en_GB
dc.date.available2011-10-26T10:35:09Z-
dc.date.issued2011-10-17en_GB
dc.date.accessioned2011-10-26T10:35:09Z-
dc.description.sponsorshipSigma Theta Tau Internationalen_GB
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