A Comparison of the Effects of Potential Chemotherapeutic Agents, Thymoquinone and Epigallocatechin-3-gallate, versus 5-Fluorouracil in a Colon Cancer Cell Line

2.50
Hdl Handle:
http://hdl.handle.net/10755/152255
Type:
Presentation
Title:
A Comparison of the Effects of Potential Chemotherapeutic Agents, Thymoquinone and Epigallocatechin-3-gallate, versus 5-Fluorouracil in a Colon Cancer Cell Line
Abstract:
A Comparison of the Effects of Potential Chemotherapeutic Agents, Thymoquinone and Epigallocatechin-3-gallate, versus 5-Fluorouracil in a Colon Cancer Cell Line
Conference Sponsor:Sigma Theta Tau International
Conference Year:2006
Author:Norwood, Anne A., RN, MSN, CS-FNP
P.I. Institution Name:University of Mississippi Medical Center School of Nursing
Title:Instructor
Co-Authors:Michelle A. Tucci, PhD; Hamed A. Benghuzzi, PhD
Antioxidants have been found to be quite successful in deterring certain disease processes for years, especially cancer.  Antioxidants protect the body by neutralizing the free radicals and donating one of their own electrons, thus ending the scavenger reaction.  Epigallocatechin-3-gallate (EGCG), the most abundant catechin found in green tea, is a valuable scavenger of reactive oxygen species in vitro as well as in vivo.  Thymoquinone (TQ), a major active component of black seed (Nigella sativa), is also known for its powerful scavenger abilities as an inhibitor of oxidative stress and has been utilized in the Middle East for centuries for healing properties.  These two potent antioxidants when compared to the chemotherapeutic drug of choice, 5-fluorouracil (5-FU), have demonstrated incredible chemotherapeutic responses to the SW-626 cell line.  The objective of this study was to evaluate and compare the effects of SW-626 colon cancer cells after a 24, 48, and 72 hour incubation periods with low, medium, and high doses of EGCG, TQ, and 5-FU.  Cell viability, cell number, cellular morphology, and cellular metabolism were compared for the control and treatment groups.  The results of this study evidenced a similar significant decrease in cell number as early as 24 hours in the groups treated with GT and EGCG compared to 5-FU.  Increases in cellular damage were evident after 24, 48, and 72 hours and in all treated groups compared with the control.  Reduced cell numbers in the treated groups suggests the possibility that GT and EGCG may have similar chemotherapeutic effects on cancer cells as 5-FU.
Repository Posting Date:
26-Oct-2011
Date of Publication:
17-Oct-2011
Sponsors:
Sigma Theta Tau International

Full metadata record

DC FieldValue Language
dc.typePresentationen_GB
dc.titleA Comparison of the Effects of Potential Chemotherapeutic Agents, Thymoquinone and Epigallocatechin-3-gallate, versus 5-Fluorouracil in a Colon Cancer Cell Lineen_GB
dc.identifier.urihttp://hdl.handle.net/10755/152255-
dc.description.abstract<table><tr><td colspan="2" class="item-title">A Comparison of the Effects of Potential Chemotherapeutic Agents, Thymoquinone and Epigallocatechin-3-gallate, versus 5-Fluorouracil in a Colon Cancer Cell Line</td></tr><tr class="item-sponsor"><td class="label">Conference Sponsor:</td><td class="value">Sigma Theta Tau International</td></tr><tr class="item-year"><td class="label">Conference Year:</td><td class="value">2006</td></tr><tr class="item-author"><td class="label">Author:</td><td class="value">Norwood, Anne A., RN, MSN, CS-FNP</td></tr><tr class="item-institute"><td class="label">P.I. Institution Name:</td><td class="value">University of Mississippi Medical Center School of Nursing</td></tr><tr class="item-author-title"><td class="label">Title:</td><td class="value">Instructor</td></tr><tr class="item-email"><td class="label">Email:</td><td class="value">anorwood@son.umsmed.edu</td></tr><tr class="item-co-authors"><td class="label">Co-Authors:</td><td class="value">Michelle A. Tucci, PhD; Hamed A. Benghuzzi, PhD</td></tr><tr><td colspan="2" class="item-abstract">Antioxidants have been found to be quite successful in deterring certain disease processes for years, especially cancer.&nbsp; Antioxidants protect the body by neutralizing the free radicals and donating one of their own electrons, thus ending the scavenger reaction.&nbsp; Epigallocatechin-3-gallate (EGCG), the most abundant catechin found in green tea, is a valuable scavenger of reactive oxygen species&nbsp;in vitro&nbsp;as well as in vivo.&nbsp; Thymoquinone (TQ), a major active component of black seed (Nigella sativa), is also known for its powerful scavenger abilities as an inhibitor of oxidative stress and has been utilized in the Middle East for centuries for healing properties.&nbsp; These two potent antioxidants when compared to the chemotherapeutic drug of choice, 5-fluorouracil (5-FU), have demonstrated incredible chemotherapeutic responses to the SW-626 cell line.&nbsp; The objective of this study was to evaluate and compare the effects of SW-626 colon cancer cells after a 24, 48, and 72 hour incubation periods with low, medium, and high doses of EGCG, TQ, and 5-FU.&nbsp; Cell viability, cell number, cellular morphology, and cellular metabolism were compared for the control and treatment groups.&nbsp; The results of this study evidenced a similar significant decrease in cell number as early as 24 hours in the groups treated with GT and EGCG compared to 5-FU.&nbsp; Increases in cellular damage were evident after 24, 48, and 72 hours and in all treated groups compared with the control.&nbsp; Reduced cell numbers in the treated groups suggests the possibility that GT and EGCG may have similar chemotherapeutic effects on cancer cells as 5-FU.</td></tr></table>en_GB
dc.date.available2011-10-26T11:29:28Z-
dc.date.issued2011-10-17en_GB
dc.date.accessioned2011-10-26T11:29:28Z-
dc.description.sponsorshipSigma Theta Tau Internationalen_GB
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