IDENTIFYING MITOCHONDRIAL PROTEINS IN PLASMA OF FATIGUE HIV-PATIENTS AND CONTROLS

2.50
Hdl Handle:
http://hdl.handle.net/10755/157292
Type:
Presentation
Title:
IDENTIFYING MITOCHONDRIAL PROTEINS IN PLASMA OF FATIGUE HIV-PATIENTS AND CONTROLS
Abstract:
IDENTIFYING MITOCHONDRIAL PROTEINS IN PLASMA OF FATIGUE HIV-PATIENTS AND CONTROLS
Conference Sponsor:Western Institute of Nursing
Conference Year:2010
Author:Voss, Joachim, RN
P.I. Institution Name:University of Washington
Title:Assistant Professor
Contact Address:Biobehavioral Nursing & Health Systems, Box 357266, Seattle, WA, 98195, USA
Co-Authors:Young Ah Goo; Caryn Morse; Joseph Kovacs; Larry Adams; David R. Goodlett
PURPOSES/AIMS: This study identified low-abundance nuclear and mitochondrial encoded proteins from healthy controls compared to fatigued HIV patients to identify ôsignature proteinsö for fatigue. Our hypothesis is that there are distinct mitochondrial and nuclear encoded proteins identified in the plasma of fatigued HIV patients compared to healthy controls.
RATIONALE/CONCEPTUAL BASIS/BACKGROUND: HIV treatments have been found to induce structural and functional changes of mitochondria in various tissues, leading to tissue damage, cell apoptosis and inflammation. We speculate that these events may contribute to the measureable release of mitochondrial proteins into the blood stream and may be related to fatigue perception.
METHODS: For this study, plasma samples of severely fatigued HIV patients (fatigue score >7, range 0-10, on the Piper Fatigue Scale) who received HIV treatment (n=10), and healthy control samples (n=10) were compared. To minimize presence of abundant proteins, we depleted the plasma of the seven most abundant proteins utilizing a Removal Column to enhance identification of low abundance proteins. Proteins were trypsinized and desalted with a solid phase extraction C18 column. Proteins were analyzed by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). Data generated with LC-ESI-MS/MS has allowed us to search for protein identifications via database search.
RESULTS: A total of 286 proteins were identified. Of those 170 were shared proteins in patients and healthy controls, while 59 were unique to healthy controls and 57 to fatigued HIV patients. We identified a total of 35 mitochondrially relevant proteins in healthy controls and 40 in HIV infected individuals. Of those 20 were unique to healthy controls, 15 unique to HIV patients and 20 were shared between both.
IMPLICATIONS: We were able to identify distinct mitochondrial and nuclear encoded proteins in the plasma of fatigued HIV patients compared to healthy controls. Further investigation is needed to understand the character and function of these proteins.
Repository Posting Date:
26-Oct-2011
Date of Publication:
17-Oct-2011
Sponsors:
Western Institute of Nursing

Full metadata record

DC FieldValue Language
dc.typePresentationen_GB
dc.titleIDENTIFYING MITOCHONDRIAL PROTEINS IN PLASMA OF FATIGUE HIV-PATIENTS AND CONTROLSen_GB
dc.identifier.urihttp://hdl.handle.net/10755/157292-
dc.description.abstract<table><tr><td colspan="2" class="item-title">IDENTIFYING MITOCHONDRIAL PROTEINS IN PLASMA OF FATIGUE HIV-PATIENTS AND CONTROLS</td></tr><tr class="item-sponsor"><td class="label">Conference Sponsor:</td><td class="value">Western Institute of Nursing</td></tr><tr class="item-year"><td class="label">Conference Year:</td><td class="value">2010</td></tr><tr class="item-author"><td class="label">Author:</td><td class="value">Voss, Joachim, RN</td></tr><tr class="item-institute"><td class="label">P.I. Institution Name:</td><td class="value">University of Washington</td></tr><tr class="item-author-title"><td class="label">Title:</td><td class="value">Assistant Professor</td></tr><tr class="item-address"><td class="label">Contact Address:</td><td class="value">Biobehavioral Nursing &amp; Health Systems, Box 357266, Seattle, WA, 98195, USA</td></tr><tr class="item-email"><td class="label">Email:</td><td class="value">vossj@u.washington.edu</td></tr><tr class="item-co-authors"><td class="label">Co-Authors:</td><td class="value">Young Ah Goo; Caryn Morse; Joseph Kovacs; Larry Adams; David R. Goodlett</td></tr><tr><td colspan="2" class="item-abstract">PURPOSES/AIMS: This study identified low-abundance nuclear and mitochondrial encoded proteins from healthy controls compared to fatigued HIV patients to identify &ocirc;signature proteins&ouml; for fatigue. Our hypothesis is that there are distinct mitochondrial and nuclear encoded proteins identified in the plasma of fatigued HIV patients compared to healthy controls. <br/>RATIONALE/CONCEPTUAL BASIS/BACKGROUND: HIV treatments have been found to induce structural and functional changes of mitochondria in various tissues, leading to tissue damage, cell apoptosis and inflammation. We speculate that these events may contribute to the measureable release of mitochondrial proteins into the blood stream and may be related to fatigue perception.<br/>METHODS: For this study, plasma samples of severely fatigued HIV patients (fatigue score &gt;7, range 0-10, on the Piper Fatigue Scale) who received HIV treatment (n=10), and healthy control samples (n=10) were compared. To minimize presence of abundant proteins, we depleted the plasma of the seven most abundant proteins utilizing a Removal Column to enhance identification of low abundance proteins. Proteins were trypsinized and desalted with a solid phase extraction C18 column. Proteins were analyzed by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). Data generated with LC-ESI-MS/MS has allowed us to search for protein identifications via database search.<br/>RESULTS: A total of 286 proteins were identified. Of those 170 were shared proteins in patients and healthy controls, while 59 were unique to healthy controls and 57 to fatigued HIV patients. We identified a total of 35 mitochondrially relevant proteins in healthy controls and 40 in HIV infected individuals. Of those 20 were unique to healthy controls, 15 unique to HIV patients and 20 were shared between both.<br/>IMPLICATIONS: We were able to identify distinct mitochondrial and nuclear encoded proteins in the plasma of fatigued HIV patients compared to healthy controls. Further investigation is needed to understand the character and function of these proteins.<br/></td></tr></table>en_GB
dc.date.available2011-10-26T19:44:25Z-
dc.date.issued2011-10-17en_GB
dc.date.accessioned2011-10-26T19:44:25Z-
dc.description.sponsorshipWestern Institute of Nursingen_GB
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