POSTHEMORRHAGE PRERESUSCITATION CONDITIONING WITH DIAZOXIDE INCREASES HSPs IN BRAIN

2.50
Hdl Handle:
http://hdl.handle.net/10755/157449
Type:
Presentation
Title:
POSTHEMORRHAGE PRERESUSCITATION CONDITIONING WITH DIAZOXIDE INCREASES HSPs IN BRAIN
Abstract:
POSTHEMORRHAGE PRERESUSCITATION CONDITIONING WITH DIAZOXIDE INCREASES HSPs IN BRAIN
Conference Sponsor:Western Institute of Nursing
Conference Year:2010
Author:Bentley, Michael Wayne, MAJ, CRNA
P.I. Institution Name:United States Army
Title:United States Army
Contact Address:4513 Ridge Canyon Road, Schertz, TX, 78154, USA
PURPOSES/AIMS:
The purpose of this investigation was to determine the capacity of diazoxide, a mitochondrial potassium ATP sensitive channel opener, as part of a post-injury resuscitation protocol to elicit the cerebral expression of cytoprotective proteins, HSP25 and HSP70 in laboratory rats that had sustained hemorrhage shock.
RATIONALE/CONCEPTUAL BASIS/BACKGROUND:
Hemorrhagic shock resulting from injury is the most common cause of traumatic death in both civilian and military settings. In addition, the majority of victims have a coinciding head injury. Resuscitative measures that are useful subsequent to hemorrhagic injury are few. As a result, our team investigated the hypothesis that diazoxide (DZ), a mitochondrial KATP channel opener, could be used during resuscitation to induce a post conditioning effect in a laboratory animal model of hemorrhagic shock with concomitant cerebral ischemic injury.
METHODS:
Male Sprague-Dawley rats underwent a 40% hemorrhage and resuscitation one hour later. Diazoxide was administered at one of three time points: 20 minutes before the onset of resuscitation, at the time that resuscitation began, or 20 minutes after the initiation of resuscitation. Western blotting and immunohistochemistry techniques were used to assess the ability of DZ to elevate the expression of the cytoprotective heat shock proteins (HSP) HSP25 and HSP70 within the cerebral cortex and hippocampus following hemorrhagic injury.
RESULTS:
When DZ was administered intravenously 20 minutes prior to resuscitative efforts following hemorrhagic shock, HSP25 and HSP70 exhibited marked up regulation.
IMPLICATIONS:
These results suggest that following a traumatic hemorrhagic injury, neuroprotection may be conferred when giving a mitochondrial potassium ATP sensitive channel opener prior to the onset of fluid resuscitation efforts.
Repository Posting Date:
26-Oct-2011
Date of Publication:
17-Oct-2011
Sponsors:
Western Institute of Nursing

Full metadata record

DC FieldValue Language
dc.typePresentationen_GB
dc.titlePOSTHEMORRHAGE PRERESUSCITATION CONDITIONING WITH DIAZOXIDE INCREASES HSPs IN BRAINen_GB
dc.identifier.urihttp://hdl.handle.net/10755/157449-
dc.description.abstract<table><tr><td colspan="2" class="item-title">POSTHEMORRHAGE PRERESUSCITATION CONDITIONING WITH DIAZOXIDE INCREASES HSPs IN BRAIN</td></tr><tr class="item-sponsor"><td class="label">Conference Sponsor:</td><td class="value">Western Institute of Nursing</td></tr><tr class="item-year"><td class="label">Conference Year:</td><td class="value">2010</td></tr><tr class="item-author"><td class="label">Author:</td><td class="value">Bentley, Michael Wayne, MAJ, CRNA</td></tr><tr class="item-institute"><td class="label">P.I. Institution Name:</td><td class="value">United States Army</td></tr><tr class="item-author-title"><td class="label">Title:</td><td class="value">United States Army</td></tr><tr class="item-address"><td class="label">Contact Address:</td><td class="value">4513 Ridge Canyon Road, Schertz, TX, 78154, USA</td></tr><tr class="item-email"><td class="label">Email:</td><td class="value">michael.bentley@us.army.mil</td></tr><tr><td colspan="2" class="item-abstract">PURPOSES/AIMS: <br/>The purpose of this investigation was to determine the capacity of diazoxide, a mitochondrial potassium ATP sensitive channel opener, as part of a post-injury resuscitation protocol to elicit the cerebral expression of cytoprotective proteins, HSP25 and HSP70 in laboratory rats that had sustained hemorrhage shock. <br/>RATIONALE/CONCEPTUAL BASIS/BACKGROUND: <br/>Hemorrhagic shock resulting from injury is the most common cause of traumatic death in both civilian and military settings. In addition, the majority of victims have a coinciding head injury. Resuscitative measures that are useful subsequent to hemorrhagic injury are few. As a result, our team investigated the hypothesis that diazoxide (DZ), a mitochondrial KATP channel opener, could be used during resuscitation to induce a post conditioning effect in a laboratory animal model of hemorrhagic shock with concomitant cerebral ischemic injury. <br/>METHODS: <br/>Male Sprague-Dawley rats underwent a 40% hemorrhage and resuscitation one hour later. Diazoxide was administered at one of three time points: 20 minutes before the onset of resuscitation, at the time that resuscitation began, or 20 minutes after the initiation of resuscitation. Western blotting and immunohistochemistry techniques were used to assess the ability of DZ to elevate the expression of the cytoprotective heat shock proteins (HSP) HSP25 and HSP70 within the cerebral cortex and hippocampus following hemorrhagic injury. <br/>RESULTS: <br/>When DZ was administered intravenously 20 minutes prior to resuscitative efforts following hemorrhagic shock, HSP25 and HSP70 exhibited marked up regulation. <br/>IMPLICATIONS: <br/>These results suggest that following a traumatic hemorrhagic injury, neuroprotection may be conferred when giving a mitochondrial potassium ATP sensitive channel opener prior to the onset of fluid resuscitation efforts.<br/></td></tr></table>en_GB
dc.date.available2011-10-26T19:53:01Z-
dc.date.issued2011-10-17en_GB
dc.date.accessioned2011-10-26T19:53:01Z-
dc.description.sponsorshipWestern Institute of Nursingen_GB
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