2.50
Hdl Handle:
http://hdl.handle.net/10755/157747
Type:
Presentation
Title:
Use of Select Proteomic Technologies
Abstract:
Use of Select Proteomic Technologies
Conference Sponsor:Western Institute of Nursing
Conference Year:2009
Author:Hastings-Tolsma, Marie, PhD, CNM
P.I. Institution Name:University of Colorado Denver, College of Nursing
Title:Associate Professor
Contact Address:Mail Stop C288-18, 13120 E. 19th Avenue, Room 4315, P.O. Box 6511, Aurora, CO, 80045, USA
Contact Telephone:303-724-8543
Background/Purpose: Proteomics is defined as the study of proteins and is the global analysis of gene expression at the protein level. It involves the study of protein pathways, expression, function, and structure through a variety of technologies and is invaluable in the identification of clinically relevant processes. The identification of proteins expressed in specific physiological states is useful for many purposes, including diagnosis. One such example is cervical remodeling which occurs across gestation in preparation for parturition - whether preterm, term, or post-dates. Understanding of proteomics related to how the cervix remodels in preparation for birth may foster discoveries that target specific protein pathways which may promote or impede cervical change. Measurement Description: A variety of technologies can be used for proteomic measurement. A sample of proteins is commonly examined by techniques such as 2-D gel electrophoresis, mass spectrometry, and microarray analysis. Gel electrophoresis is a process of separating molecules (proteins, DNA, RNA fragments) by size and electrical charge. Mass spectrometry is used to identify proteins in a substance by their mass and charge. Subsequent techniques can then be used to isolate proteins and identify their characteristics. Protein microarray analysis is a method of profiling protein changes in a diseased or physiologic state. It allows for examination of thousands of mRNAs at one time and determination as to whether mRNA expression is changed in differing physiologic and pathologic states. Each of these measurement methods aids in differentiating between physiological processes and diseased states. These analytic approaches can be very expensive and time consuming, but access to core facilities may reduce fees and ensure robust methods that demonstrate a high sensitivity and are reproducible. The yield of an individual protein depends on the conditions for sample preparation. Small changes result in a different sample composition and another set of proteins. Identifying proteins that are disease-specific and/or specific to a physiologic state may contribute to the subsequent development of biomarkers predictive for diseases or states that need treatment. Applications: Science progression has focused heavily on proteins, the main effectors of physiological functions. Newer proteomic technologies have made it possible to improve patient care in an increasingly personal way. Applications are unlimited and range from detection of diabetes and pre-diabetes, to Parkinson's disease, mental health disorders such as schizophrenia, and inflammatory processes (e.g., rheumatoid arthritis, brain injury). Detecting differences in protein levels and modifications which exist between healthy and diseased states are critical and necessary for development of preventive and predictive health models. Application of proteomic technologies in the investigation of specific diseases, pathologic processes, and physiologic states are important in furthering knowledge to improve patient care. Conclusions: Nurse researchers are well-positioned for independent and collaborative work involving proteomics, which may ultimately lead to the use of protein markers that may improve diagnoses and treatment plans.
Repository Posting Date:
26-Oct-2011
Date of Publication:
17-Oct-2011
Sponsors:
Western Institute of Nursing

Full metadata record

DC FieldValue Language
dc.typePresentationen_GB
dc.titleUse of Select Proteomic Technologiesen_GB
dc.identifier.urihttp://hdl.handle.net/10755/157747-
dc.description.abstract<table><tr><td colspan="2" class="item-title">Use of Select Proteomic Technologies</td></tr><tr class="item-sponsor"><td class="label">Conference Sponsor:</td><td class="value">Western Institute of Nursing</td></tr><tr class="item-year"><td class="label">Conference Year:</td><td class="value">2009</td></tr><tr class="item-author"><td class="label">Author:</td><td class="value">Hastings-Tolsma, Marie, PhD, CNM</td></tr><tr class="item-institute"><td class="label">P.I. Institution Name:</td><td class="value">University of Colorado Denver, College of Nursing</td></tr><tr class="item-author-title"><td class="label">Title:</td><td class="value">Associate Professor</td></tr><tr class="item-address"><td class="label">Contact Address:</td><td class="value">Mail Stop C288-18, 13120 E. 19th Avenue, Room 4315, P.O. Box 6511, Aurora, CO, 80045, USA</td></tr><tr class="item-phone"><td class="label">Contact Telephone:</td><td class="value">303-724-8543</td></tr><tr class="item-email"><td class="label">Email:</td><td class="value">Marie.Hastings-Tolsma@ucdenver.edu</td></tr><tr><td colspan="2" class="item-abstract">Background/Purpose: Proteomics is defined as the study of proteins and is the global analysis of gene expression at the protein level. It involves the study of protein pathways, expression, function, and structure through a variety of technologies and is invaluable in the identification of clinically relevant processes. The identification of proteins expressed in specific physiological states is useful for many purposes, including diagnosis. One such example is cervical remodeling which occurs across gestation in preparation for parturition - whether preterm, term, or post-dates. Understanding of proteomics related to how the cervix remodels in preparation for birth may foster discoveries that target specific protein pathways which may promote or impede cervical change. Measurement Description: A variety of technologies can be used for proteomic measurement. A sample of proteins is commonly examined by techniques such as 2-D gel electrophoresis, mass spectrometry, and microarray analysis. Gel electrophoresis is a process of separating molecules (proteins, DNA, RNA fragments) by size and electrical charge. Mass spectrometry is used to identify proteins in a substance by their mass and charge. Subsequent techniques can then be used to isolate proteins and identify their characteristics. Protein microarray analysis is a method of profiling protein changes in a diseased or physiologic state. It allows for examination of thousands of mRNAs at one time and determination as to whether mRNA expression is changed in differing physiologic and pathologic states. Each of these measurement methods aids in differentiating between physiological processes and diseased states. These analytic approaches can be very expensive and time consuming, but access to core facilities may reduce fees and ensure robust methods that demonstrate a high sensitivity and are reproducible. The yield of an individual protein depends on the conditions for sample preparation. Small changes result in a different sample composition and another set of proteins. Identifying proteins that are disease-specific and/or specific to a physiologic state may contribute to the subsequent development of biomarkers predictive for diseases or states that need treatment. Applications: Science progression has focused heavily on proteins, the main effectors of physiological functions. Newer proteomic technologies have made it possible to improve patient care in an increasingly personal way. Applications are unlimited and range from detection of diabetes and pre-diabetes, to Parkinson's disease, mental health disorders such as schizophrenia, and inflammatory processes (e.g., rheumatoid arthritis, brain injury). Detecting differences in protein levels and modifications which exist between healthy and diseased states are critical and necessary for development of preventive and predictive health models. Application of proteomic technologies in the investigation of specific diseases, pathologic processes, and physiologic states are important in furthering knowledge to improve patient care. Conclusions: Nurse researchers are well-positioned for independent and collaborative work involving proteomics, which may ultimately lead to the use of protein markers that may improve diagnoses and treatment plans.</td></tr></table>en_GB
dc.date.available2011-10-26T20:09:56Z-
dc.date.issued2011-10-17en_GB
dc.date.accessioned2011-10-26T20:09:56Z-
dc.description.sponsorshipWestern Institute of Nursingen_GB
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