2.50
Hdl Handle:
http://hdl.handle.net/10755/157802
Type:
Presentation
Title:
Multiplex Assays: 1 vs. 100 Cytokine Measures
Abstract:
Multiplex Assays: 1 vs. 100 Cytokine Measures
Conference Sponsor:Western Institute of Nursing
Conference Year:2009
Author:McCain, Nancy L., RN, DSN, FAAN
P.I. Institution Name:Virginia Commonwealth University, School of Nursing
Title:Director, Center for Biobehavioral Clinical Research (CBCR)
Contact Address:1100 East Leigh Street, PO Box 980567, Richmond, VA, 23298, USA
Contact Telephone:804-828-3444
Purpose/Background: The psychoneuroimmunology (PNI) paradigm postulates multidimensional interactions among psychosocial factors and physiological mechanisms of the neuroendocrine and immune systems. It is known, for example, that the stress response involves both direct and indirect effects on the immune system, such that numerous cytokines and other biochemicals such as endogenous opioids are elaborated by cells of the immune system. Given recent technological advancements, state-of-the-science and emerging analytic approaches to evaluate patterns of cytokines can be employed to discern meaningful effects and potential biobehavioral mechanisms. Measurement Description: Past investigations of cytokines have been limited to relatively few cytokines studied in isolation, in part because of technical limitations of biological analyses. Cytokines have been traditionally measured using enzyme-linked immunosorbent assays (ELISA), wherein levels of an individual cytokine protein are measured after binding to its specific antibody affixed in a 96-well plate. Using Luminex xMAPTM technology, up to 100 different analytes can now be simultaneously measured in a single microtiter well. Systems such as the Bio-Plex (Bio-Rad Laboratories) employ fluorescent bead immunoassays to simultaneously quantitate multiple cytokines. The Bio- Plex instrument combines fluorescent flow cytometry and ELISA technology. For each measured cytokine, 100 microbeads specific for that cytokine are sampled and the mean cytokine binding for the sample is determined. The assay accurately measures cytokine values in the range of 1-2,500 pg/ml, is precise (intra-assay CV <10%, interassay CV <15%), and shows less than 1% cross-reactivity among cytokines or with other molecules. While the initial investment in the instrument is considerable, multiple assays and automated systems ultimately result in reduced costs for multiplex assays in comparison to single ELISA assays of cytokines. Applications: Approaches for integrating complex biobehavioral datasets are being developed in order to advance interpretation and clinical applications of these multidimensional data. Our research programs have demonstrated, for example, quite diverse patterns of cytokines for persons with HIV infection in comparison to women being treated for breast cancer. Although levels of individual cytokines indicate some differences between the groups, more integrative approaches such as factor analysis and profile plots reveal dramatic pattern differences between the groups when sets of cytokines are simultaneously analyzed. Such analytic approaches will ultimately enable measurement, identification, and interpretation of complex patterns among cytokines, and thus, illuminate underlying mechanisms.
Repository Posting Date:
26-Oct-2011
Date of Publication:
17-Oct-2011
Sponsors:
Western Institute of Nursing

Full metadata record

DC FieldValue Language
dc.typePresentationen_GB
dc.titleMultiplex Assays: 1 vs. 100 Cytokine Measuresen_GB
dc.identifier.urihttp://hdl.handle.net/10755/157802-
dc.description.abstract<table><tr><td colspan="2" class="item-title">Multiplex Assays: 1 vs. 100 Cytokine Measures</td></tr><tr class="item-sponsor"><td class="label">Conference Sponsor:</td><td class="value">Western Institute of Nursing</td></tr><tr class="item-year"><td class="label">Conference Year:</td><td class="value">2009</td></tr><tr class="item-author"><td class="label">Author:</td><td class="value">McCain, Nancy L., RN, DSN, FAAN</td></tr><tr class="item-institute"><td class="label">P.I. Institution Name:</td><td class="value">Virginia Commonwealth University, School of Nursing</td></tr><tr class="item-author-title"><td class="label">Title:</td><td class="value">Director, Center for Biobehavioral Clinical Research (CBCR)</td></tr><tr class="item-address"><td class="label">Contact Address:</td><td class="value">1100 East Leigh Street, PO Box 980567, Richmond, VA, 23298, USA</td></tr><tr class="item-phone"><td class="label">Contact Telephone:</td><td class="value">804-828-3444</td></tr><tr class="item-email"><td class="label">Email:</td><td class="value">nlmccain@vcu.edu</td></tr><tr><td colspan="2" class="item-abstract">Purpose/Background: The psychoneuroimmunology (PNI) paradigm postulates multidimensional interactions among psychosocial factors and physiological mechanisms of the neuroendocrine and immune systems. It is known, for example, that the stress response involves both direct and indirect effects on the immune system, such that numerous cytokines and other biochemicals such as endogenous opioids are elaborated by cells of the immune system. Given recent technological advancements, state-of-the-science and emerging analytic approaches to evaluate patterns of cytokines can be employed to discern meaningful effects and potential biobehavioral mechanisms. Measurement Description: Past investigations of cytokines have been limited to relatively few cytokines studied in isolation, in part because of technical limitations of biological analyses. Cytokines have been traditionally measured using enzyme-linked immunosorbent assays (ELISA), wherein levels of an individual cytokine protein are measured after binding to its specific antibody affixed in a 96-well plate. Using Luminex xMAPTM technology, up to 100 different analytes can now be simultaneously measured in a single microtiter well. Systems such as the Bio-Plex (Bio-Rad Laboratories) employ fluorescent bead immunoassays to simultaneously quantitate multiple cytokines. The Bio- Plex instrument combines fluorescent flow cytometry and ELISA technology. For each measured cytokine, 100 microbeads specific for that cytokine are sampled and the mean cytokine binding for the sample is determined. The assay accurately measures cytokine values in the range of 1-2,500 pg/ml, is precise (intra-assay CV &lt;10%, interassay CV &lt;15%), and shows less than 1% cross-reactivity among cytokines or with other molecules. While the initial investment in the instrument is considerable, multiple assays and automated systems ultimately result in reduced costs for multiplex assays in comparison to single ELISA assays of cytokines. Applications: Approaches for integrating complex biobehavioral datasets are being developed in order to advance interpretation and clinical applications of these multidimensional data. Our research programs have demonstrated, for example, quite diverse patterns of cytokines for persons with HIV infection in comparison to women being treated for breast cancer. Although levels of individual cytokines indicate some differences between the groups, more integrative approaches such as factor analysis and profile plots reveal dramatic pattern differences between the groups when sets of cytokines are simultaneously analyzed. Such analytic approaches will ultimately enable measurement, identification, and interpretation of complex patterns among cytokines, and thus, illuminate underlying mechanisms.</td></tr></table>en_GB
dc.date.available2011-10-26T20:13:08Z-
dc.date.issued2011-10-17en_GB
dc.date.accessioned2011-10-26T20:13:08Z-
dc.description.sponsorshipWestern Institute of Nursingen_GB
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