Building Knowledge About the Menopausal Transition: The SMWHS Project, Genetic Influences on Cortisol Level During Late Menopause Transition

2.50
Hdl Handle:
http://hdl.handle.net/10755/158002
Type:
Presentation
Title:
Building Knowledge About the Menopausal Transition: The SMWHS Project, Genetic Influences on Cortisol Level During Late Menopause Transition
Abstract:
Building Knowledge About the Menopausal Transition: The SMWHS Project, Genetic Influences on Cortisol Level During Late Menopause Transition
Conference Sponsor:Western Institute of Nursing
Conference Year:2005
Author:Woods, Nancy, PhD, RN, FAAN
P.I. Institution Name:University of Washington School of Nursing
Title:Professor and Dean
Contact Address:Box 357260, Seattle, WA, 98195-7260, USA
Contact Telephone:206-221-2472
Co-Authors:Ellen S. Mitchell, Yun Tao, Molly Carr, Federico Farin, Hannah A. Viernes, Patricia L. Stapleton
1. Background: Women in the SWAN study experience a transient rise in adrenal androgens (DHEAS) during the late menopausal transition stage and recently we have noted a rise in cortisol among women in the Seattle Midlife Women's Health Cohort during this stage.áOthers have noted an association between cortisol levels and CYP 19 polymorphisms. Purpose:áThe purpose of this study was to determine whether aárise in cortisol andá cortisol levels during the menopausal transition are related to CYP 19 polymorphisms, in particular CYP 19 7r and CYP 19 7r(-3). Methods:áParticipants in the Seattle Midlife Women's Health Study since 1990 provided a buccal smear in 2002 for genotyping (N=174) in addition to their daily menstrual calendars, symptom diaries, and monthly early am urine samples assayed for cortisol. Data were analyzed for women in the middle (n=67), late (59) menopausal transition stages or early postmenopause (34) had provided buccal smears for genotyping and urine samples. Genotyping was completed for the following polymorphisms:á CYP 1A1m2, CYP 1A1m4, CYP 1B1*2, CYP 1B1*3, CYP 17 5'UTR (A1/A2), CYP 19 3'UTR (C/T), CYP 19 (TTTA)n, ESR1XbaI, ESR1PvuII. Menstrual calendars were used for staging the menopausal transition. Results:áCortisol levels rose from the middle to late menopausal transition stages (p<.05).á Of the polymorphisms in the estrogen synthesis pathways, only CYP 19 7r and CYP 19 7r(-3) were significantly associated with a cortisol rise during the late menopausal transition stage.á Women with a cortisol rise of 10ng/mg creatinine were less likely to have 1 or 2 repeats of CYP 19 7r (p<.05) and were more likely to have 1 or 2 repeats of CYP 19 7r(-3) (P<.05). Analysis of variance indicated that women with one or two copies of the CYP 19 7r or CYP 19 7r(-3) polymorphisms did not have significantly different cortisol levels.áHowever, women with one or two copies of the CYP 1B1*2 had significantly higher levels of cortisol during the middle menopausal transition stage.á Those with the ESRXbaI polymorphism had significantly lower cortisol levels during the middle menopausal transition stage.áDiscussion:áCYP 19 7r and CYP19 7r(-3) were significantly associated with a cortisol rise during the same stage of the menopausal transition in which others have observed a rise in DHEAS.áMoreover, this finding is consistent with that from Baghei and associates that women with a short microsatellite in the fourth intron of the CYP 19 gene caused by a TCT deletion upstream had higher late morning and higher bedtime cortisol levels that did not unwind as fast as women without the polymorphism. These women also had higher androgen levels and central obesity. Associations of the CYP 1b1*2 and ESRXbaI polymorphisms and cortisol levels may reflect the influence of estrogen metabolism on cortisol levels.á Replication of these findings in larger studies will be important to clarify the role of aromatase cytochrome P450 polymorphisms in cortisol metabolism during the menopausal transition.
Repository Posting Date:
26-Oct-2011
Date of Publication:
17-Oct-2011
Sponsors:
Western Institute of Nursing

Full metadata record

DC FieldValue Language
dc.typePresentationen_GB
dc.titleBuilding Knowledge About the Menopausal Transition: The SMWHS Project, Genetic Influences on Cortisol Level During Late Menopause Transitionen_GB
dc.identifier.urihttp://hdl.handle.net/10755/158002-
dc.description.abstract<table><tr><td colspan="2" class="item-title">Building Knowledge About the Menopausal Transition: The SMWHS Project, Genetic Influences on Cortisol Level During Late Menopause Transition</td></tr><tr class="item-sponsor"><td class="label">Conference Sponsor:</td><td class="value">Western Institute of Nursing</td></tr><tr class="item-year"><td class="label">Conference Year:</td><td class="value">2005</td></tr><tr class="item-author"><td class="label">Author:</td><td class="value">Woods, Nancy, PhD, RN, FAAN</td></tr><tr class="item-institute"><td class="label">P.I. Institution Name:</td><td class="value">University of Washington School of Nursing</td></tr><tr class="item-author-title"><td class="label">Title:</td><td class="value">Professor and Dean</td></tr><tr class="item-address"><td class="label">Contact Address:</td><td class="value">Box 357260, Seattle, WA, 98195-7260, USA</td></tr><tr class="item-phone"><td class="label">Contact Telephone:</td><td class="value">206-221-2472</td></tr><tr class="item-email"><td class="label">Email:</td><td class="value">nfwoods@u.washington.edu</td></tr><tr class="item-co-authors"><td class="label">Co-Authors:</td><td class="value">Ellen S. Mitchell, Yun Tao, Molly Carr, Federico Farin, Hannah A. Viernes, Patricia L. Stapleton</td></tr><tr><td colspan="2" class="item-abstract">1. Background: Women in the SWAN study experience a transient rise in adrenal androgens (DHEAS) during the late menopausal transition stage and recently we have noted a rise in cortisol among women in the Seattle Midlife Women's Health Cohort during this stage.&aacute;Others have noted an association between cortisol levels and CYP 19 polymorphisms. Purpose:&aacute;The purpose of this study was to determine whether a&aacute;rise in cortisol and&aacute; cortisol levels during the menopausal transition are related to CYP 19 polymorphisms, in particular CYP 19 7r and CYP 19 7r(-3). Methods:&aacute;Participants in the Seattle Midlife Women's Health Study since 1990 provided a buccal smear in 2002 for genotyping (N=174) in addition to their daily menstrual calendars, symptom diaries, and monthly early am urine samples assayed for cortisol. Data were analyzed for women in the middle (n=67), late (59) menopausal transition stages or early postmenopause (34) had provided buccal smears for genotyping and urine samples. Genotyping was completed for the following polymorphisms:&aacute; CYP 1A1m2, CYP 1A1m4, CYP 1B1*2, CYP 1B1*3, CYP 17 5'UTR (A1/A2), CYP 19 3'UTR (C/T), CYP 19 (TTTA)n, ESR1XbaI, ESR1PvuII. Menstrual calendars were used for staging the menopausal transition. Results:&aacute;Cortisol levels rose from the middle to late menopausal transition stages (p&lt;.05).&aacute; Of the polymorphisms in the estrogen synthesis pathways, only CYP 19 7r and CYP 19 7r(-3) were significantly associated with a cortisol rise during the late menopausal transition stage.&aacute; Women with a cortisol rise of 10ng/mg creatinine were less likely to have 1 or 2 repeats of CYP 19 7r (p&lt;.05) and were more likely to have 1 or 2 repeats of CYP 19 7r(-3) (P&lt;.05). Analysis of variance indicated that women with one or two copies of the CYP 19 7r or CYP 19 7r(-3) polymorphisms did not have significantly different cortisol levels.&aacute;However, women with one or two copies of the CYP 1B1*2 had significantly higher levels of cortisol during the middle menopausal transition stage.&aacute; Those with the ESRXbaI polymorphism had significantly lower cortisol levels during the middle menopausal transition stage.&aacute;Discussion:&aacute;CYP 19 7r and CYP19 7r(-3) were significantly associated with a cortisol rise during the same stage of the menopausal transition in which others have observed a rise in DHEAS.&aacute;Moreover, this finding is consistent with that from Baghei and associates that women with a short microsatellite in the fourth intron of the CYP 19 gene caused by a TCT deletion upstream had higher late morning and higher bedtime cortisol levels that did not unwind as fast as women without the polymorphism. These women also had higher androgen levels and central obesity. Associations of the CYP 1b1*2 and ESRXbaI polymorphisms and cortisol levels may reflect the influence of estrogen metabolism on cortisol levels.&aacute; Replication of these findings in larger studies will be important to clarify the role of aromatase cytochrome P450 polymorphisms in cortisol metabolism during the menopausal transition.</td></tr></table>en_GB
dc.date.available2011-10-26T20:24:46Z-
dc.date.issued2011-10-17en_GB
dc.date.accessioned2011-10-26T20:24:46Z-
dc.description.sponsorshipWestern Institute of Nursingen_GB
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