HIV- and HPV-Infection Characteristics for a Sample of Homosexual Men Evaluated Using High Resolution Anoscopy

2.50
Hdl Handle:
http://hdl.handle.net/10755/158122
Type:
Presentation
Title:
HIV- and HPV-Infection Characteristics for a Sample of Homosexual Men Evaluated Using High Resolution Anoscopy
Abstract:
HIV- and HPV-Infection Characteristics for a Sample of Homosexual Men Evaluated Using High Resolution Anoscopy
Conference Sponsor:Western Institute of Nursing
Conference Year:2004
Author:Wiley, D., PhD, MPH, RN
P.I. Institution Name:Unviersity of California-Los Angeles
Contact Address:700 Tiverton Ve, Suite 5-151, Mail Code 691921, Los Angeles, CA, 90095-6919, USA
Co-Authors:Rao JY; Tang SJ; Chang C; Goetz M; Boscardin J; Wiesmeier E; Detels R; Masongsong E; Chang CI
Background: Anal squamous intraepithelial lesions (SILs) and squamous cell carcinomas (SCCs) at or near the junction between the anal squamous epithelium are an important public health problem and are likely to be HPV-related. Although anal SCCs are rare generally, they will likely increase as HIV-infected men and women survive longer with effective HIV-therapy. Data suggests that premalignant anal lesions are common among homosexual men and some analyses suggest that cellular immunity are important predictors of (HPV) clearance. However, recent data suggest HIV-related highly active antiretroviral therapy may be negatively associated with clearance of anogenital HPV-related SILSs and positively associated with progression. Objective: To assess the HPV and HIV infection characteristics of 53 HIV-infected men evaluated using Pap test, high resolution anoscopy and medical biopsy, where indicated. Also, the number of lesion/tissue-infiltrating lymphocytes and inflammatory cytokines were assessed for twelve participants: 6 with high-grade SIL (HSIL)/SCC, and six randomly selected men who evidenced low-grade SIL (LSIL). Design: Cross-sectional analysis of study group characteristics and in-depth analysis of 12 biopsy specimens using immunohistochemistry. Comparisons were made between HSIL/SCC specimens and LSIL specimens and between each man's biopsy specimen and his own (negative) control specimen. Setting and Subjects: Fifty-five examinations of 53 men recruited from the Multicenter Cohort Study and the Greater Los Angeles Veteran's Administration Hospital and Medical Center. Results: Intra-anal HPV infection was common; only one man was uninfected, 8 were infected with a single Type and 20 tested positive for HPV-16. Thirteen (24%) men showed HSIL on Pap test and 15 (27%) showed no identifiable lesions (NIL). For 28 (51%) men, high-grade anal intraepithelial neoplasia was evident on biopsy and only 8 showed NIL. Immunohistochemistry analyses of twelve HSIL/ or LSIL/negative control sets showed few tissue-infiltrating lymphocytes (TILSs) within lesions or the respective negative control specimens. However, TILs often underlay HSILs/SCCs and LSILs (p<0.01). Quantitative PCR for eight proinflammatory cytokines showed no real differences between a man's lesion and his negative control specimen. Conclusions: These data suggest that, for HIV infected men, multitype infection may be common, as might intra-anal atypias. HIV-related anal HSIL/SCC/LSILs showed fewer TILs than in juxtaposed supporting stromal tissues; suggesting that either homing to virus-effected tissues or antigen-specific responses may be blunted or lost. This is a pilot study using few samples and these findings should be evaluated with caution.
Repository Posting Date:
26-Oct-2011
Date of Publication:
17-Oct-2011
Sponsors:
Western Institute of Nursing

Full metadata record

DC FieldValue Language
dc.typePresentationen_GB
dc.titleHIV- and HPV-Infection Characteristics for a Sample of Homosexual Men Evaluated Using High Resolution Anoscopyen_GB
dc.identifier.urihttp://hdl.handle.net/10755/158122-
dc.description.abstract<table><tr><td colspan="2" class="item-title">HIV- and HPV-Infection Characteristics for a Sample of Homosexual Men Evaluated Using High Resolution Anoscopy</td></tr><tr class="item-sponsor"><td class="label">Conference Sponsor:</td><td class="value">Western Institute of Nursing</td></tr><tr class="item-year"><td class="label">Conference Year:</td><td class="value">2004</td></tr><tr class="item-author"><td class="label">Author:</td><td class="value">Wiley, D., PhD, MPH, RN </td></tr><tr class="item-institute"><td class="label">P.I. Institution Name:</td><td class="value">Unviersity of California-Los Angeles</td></tr><tr class="item-address"><td class="label">Contact Address:</td><td class="value">700 Tiverton Ve, Suite 5-151, Mail Code 691921, Los Angeles, CA, 90095-6919, USA</td></tr><tr class="item-co-authors"><td class="label">Co-Authors:</td><td class="value">Rao JY; Tang SJ; Chang C; Goetz M; Boscardin J; Wiesmeier E; Detels R; Masongsong E; Chang CI</td></tr><tr><td colspan="2" class="item-abstract">Background: Anal squamous intraepithelial lesions (SILs) and squamous cell carcinomas (SCCs) at or near the junction between the anal squamous epithelium are an important public health problem and are likely to be HPV-related. Although anal SCCs are rare generally, they will likely increase as HIV-infected men and women survive longer with effective HIV-therapy. Data suggests that premalignant anal lesions are common among homosexual men and some analyses suggest that cellular immunity are important predictors of (HPV) clearance. However, recent data suggest HIV-related highly active antiretroviral therapy may be negatively associated with clearance of anogenital HPV-related SILSs and positively associated with progression. Objective: To assess the HPV and HIV infection characteristics of 53 HIV-infected men evaluated using Pap test, high resolution anoscopy and medical biopsy, where indicated. Also, the number of lesion/tissue-infiltrating lymphocytes and inflammatory cytokines were assessed for twelve participants: 6 with high-grade SIL (HSIL)/SCC, and six randomly selected men who evidenced low-grade SIL (LSIL). Design: Cross-sectional analysis of study group characteristics and in-depth analysis of 12 biopsy specimens using immunohistochemistry. Comparisons were made between HSIL/SCC specimens and LSIL specimens and between each man's biopsy specimen and his own (negative) control specimen. Setting and Subjects: Fifty-five examinations of 53 men recruited from the Multicenter Cohort Study and the Greater Los Angeles Veteran's Administration Hospital and Medical Center. Results: Intra-anal HPV infection was common; only one man was uninfected, 8 were infected with a single Type and 20 tested positive for HPV-16. Thirteen (24%) men showed HSIL on Pap test and 15 (27%) showed no identifiable lesions (NIL). For 28 (51%) men, high-grade anal intraepithelial neoplasia was evident on biopsy and only 8 showed NIL. Immunohistochemistry analyses of twelve HSIL/ or LSIL/negative control sets showed few tissue-infiltrating lymphocytes (TILSs) within lesions or the respective negative control specimens. However, TILs often underlay HSILs/SCCs and LSILs (p&lt;0.01). Quantitative PCR for eight proinflammatory cytokines showed no real differences between a man's lesion and his negative control specimen. Conclusions: These data suggest that, for HIV infected men, multitype infection may be common, as might intra-anal atypias. HIV-related anal HSIL/SCC/LSILs showed fewer TILs than in juxtaposed supporting stromal tissues; suggesting that either homing to virus-effected tissues or antigen-specific responses may be blunted or lost. This is a pilot study using few samples and these findings should be evaluated with caution.</td></tr></table>en_GB
dc.date.available2011-10-26T20:31:52Z-
dc.date.issued2011-10-17en_GB
dc.date.accessioned2011-10-26T20:31:52Z-
dc.description.sponsorshipWestern Institute of Nursingen_GB
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