2.50
Hdl Handle:
http://hdl.handle.net/10755/158885
Type:
Presentation
Title:
Non-overt Cardiomyopathy in a Mouse Model of Cancer Cachexia
Abstract:
Non-overt Cardiomyopathy in a Mouse Model of Cancer Cachexia
Conference Sponsor:Midwest Nursing Research Society
Conference Year:2010
Author:McCarthy Beckett, Donna, PhD
P.I. Institution Name:The Ohio State University
Contact Address:1585 Neil Ave., Columbus, OH, 43210, USA
Contact Telephone:614-292-2356
Co-Authors:D. McCarthy Beckett, D. Crawford, H. Xu, College of Nursing, The Ohio State University, Columbus, OH;
Cancer cachexia is a syndrome of weight loss, muscle wasting, weakness and fatigue that occurs in patients with advanced disease. Protein degradation in skeletal muscle is a major factor contributing to muscle wasting; the reduced muscle mass contributes to fatigue. However, no studies have been done to determine if muscle protein degradation also occurs in the myocardium. In the present study, we examined expression of MAFbx, Bnip3, and IL-6 mRNA, three biomarkers of muscle protein degradation, in the gastrocnemius and heart muscle of tumor-bearing mice. Mice were inoculated subcutaneously on the back with Colon26 adenocarinoma cells, and were sacrificed 21 days later. The gastrocnemius and heart muscles were removed and weighed, and homogenized in Trizol for extraction of total RNA. The cDNA transripts were subjected to real time PCR, and data were normalized to expression of a stable gene, GAPDH. We report that the gastrocnemius muscle, but not the heart muscle, was smaller in the tumor-bearing compared to healthy control mice. However, expression of MAFbx, Bnip3 and Il-6 mRNA were elevated in both the gastrocnemius and heart muscle of tumor-bearing mice. These data suggest that non-overt cardiomyopathy could contribute to the experience of fatigue in cancer patients.
Repository Posting Date:
26-Oct-2011
Date of Publication:
17-Oct-2011
Sponsors:
Midwest Nursing Research Society

Full metadata record

DC FieldValue Language
dc.typePresentationen_GB
dc.titleNon-overt Cardiomyopathy in a Mouse Model of Cancer Cachexiaen_GB
dc.identifier.urihttp://hdl.handle.net/10755/158885-
dc.description.abstract<table><tr><td colspan="2" class="item-title">Non-overt Cardiomyopathy in a Mouse Model of Cancer Cachexia</td></tr><tr class="item-sponsor"><td class="label">Conference Sponsor:</td><td class="value">Midwest Nursing Research Society</td></tr><tr class="item-year"><td class="label">Conference Year:</td><td class="value">2010</td></tr><tr class="item-author"><td class="label">Author:</td><td class="value">McCarthy Beckett, Donna, PhD</td></tr><tr class="item-institute"><td class="label">P.I. Institution Name:</td><td class="value">The Ohio State University</td></tr><tr class="item-address"><td class="label">Contact Address:</td><td class="value">1585 Neil Ave., Columbus, OH, 43210, USA</td></tr><tr class="item-phone"><td class="label">Contact Telephone:</td><td class="value">614-292-2356</td></tr><tr class="item-email"><td class="label">Email:</td><td class="value">mccarthy.225@osu.edu</td></tr><tr class="item-co-authors"><td class="label">Co-Authors:</td><td class="value">D. McCarthy Beckett, D. Crawford, H. Xu, College of Nursing, The Ohio State University, Columbus, OH;</td></tr><tr><td colspan="2" class="item-abstract">Cancer cachexia is a syndrome of weight loss, muscle wasting, weakness and fatigue that occurs in patients with advanced disease. Protein degradation in skeletal muscle is a major factor contributing to muscle wasting; the reduced muscle mass contributes to fatigue. However, no studies have been done to determine if muscle protein degradation also occurs in the myocardium. In the present study, we examined expression of MAFbx, Bnip3, and IL-6 mRNA, three biomarkers of muscle protein degradation, in the gastrocnemius and heart muscle of tumor-bearing mice. Mice were inoculated subcutaneously on the back with Colon26 adenocarinoma cells, and were sacrificed 21 days later. The gastrocnemius and heart muscles were removed and weighed, and homogenized in Trizol for extraction of total RNA. The cDNA transripts were subjected to real time PCR, and data were normalized to expression of a stable gene, GAPDH. We report that the gastrocnemius muscle, but not the heart muscle, was smaller in the tumor-bearing compared to healthy control mice. However, expression of MAFbx, Bnip3 and Il-6 mRNA were elevated in both the gastrocnemius and heart muscle of tumor-bearing mice. These data suggest that non-overt cardiomyopathy could contribute to the experience of fatigue in cancer patients.</td></tr></table>en_GB
dc.date.available2011-10-26T21:29:30Z-
dc.date.issued2011-10-17en_GB
dc.date.accessioned2011-10-26T21:29:30Z-
dc.description.sponsorshipMidwest Nursing Research Societyen_GB
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