2.50
Hdl Handle:
http://hdl.handle.net/10755/158899
Type:
Presentation
Title:
Clock Gene Expression in C57BL/6J and DBA/2J mice in Response to Acute Pain
Abstract:
Clock Gene Expression in C57BL/6J and DBA/2J mice in Response to Acute Pain
Conference Sponsor:Midwest Nursing Research Society
Conference Year:2010
Author:Rasmussen, Natalie
P.I. Institution Name:National Institutes of Health/National Institute of Nursing Research
Contact Address:10 Center Drive, 10 CRC/2-1339, Bethesda, MD, 20882, USA
Contact Telephone:301-443-5061
Co-Authors:N. Rasmussen, , National Institutes of Health/National Institute of Nursing Research, Bethesda, MD; C. Chaperon, P. Lane, W. Langer, K. Devish, A. Sachs, , University of Nebraska Medical Center, Omaha, NE;
Time of day variation in pain sensitivity is evident in practice. This variability may be due to the interaction of temporal and genetic factors. Of particular interest, are genes that are involved in the regulation of biological rhythms. Little is known about the role of circadian locomotor output cycles kaput (Clock) in the time of day variation in pain sensitivity. The objective of this study was to determine the contribution of time of day and genetic or individual variation to the modulation of Clock gene expression in the suprachiasmatic nucleus (SCN) of the brain in response to a painful stimulus. In the study, 8-week old, male, C57BL/6J and DBA/2J mice (n = 22-23 mice/strain) were used. The mice were maintained on a 12:12 light/dark cycle (lights on at 0600). Food and water were available ad libitum. The mice were acclimated to their environment for 1 week prior to exposing the mice to a painful stimulus, a hot-plate. After exposure to a hot-plate at six time points per day including 0000, 0400, 0800, 1200, 1600, and 2000 (n = 3-4 mice/strain/time point), brain tissue was collected for gene expression analysis. Collection of tissue timed for the dark phase (0000, 0400, and 2000) was performed under dim red light. Gene expression analysis was conducted using real-time polymerase chain reaction (RT-PCR). Data were analyzed using ANOVA. Overall, the C57BL/6J mice expressed less Clock in the SCN when compared to the DBA/2J mice. Time of day differences in Clock expression also were noted. Understanding the temporal and genetic variation in the response to pain is important as this knowledge may ultimately lead to the development of assessment and treatment strategies that are most effective and appropriate for each individual at varying times during a 24-hour day.
Repository Posting Date:
26-Oct-2011
Date of Publication:
17-Oct-2011
Sponsors:
Midwest Nursing Research Society

Full metadata record

DC FieldValue Language
dc.typePresentationen_GB
dc.titleClock Gene Expression in C57BL/6J and DBA/2J mice in Response to Acute Painen_GB
dc.identifier.urihttp://hdl.handle.net/10755/158899-
dc.description.abstract<table><tr><td colspan="2" class="item-title">Clock Gene Expression in C57BL/6J and DBA/2J mice in Response to Acute Pain</td></tr><tr class="item-sponsor"><td class="label">Conference Sponsor:</td><td class="value">Midwest Nursing Research Society</td></tr><tr class="item-year"><td class="label">Conference Year:</td><td class="value">2010</td></tr><tr class="item-author"><td class="label">Author:</td><td class="value">Rasmussen, Natalie</td></tr><tr class="item-institute"><td class="label">P.I. Institution Name:</td><td class="value">National Institutes of Health/National Institute of Nursing Research</td></tr><tr class="item-address"><td class="label">Contact Address:</td><td class="value">10 Center Drive, 10 CRC/2-1339, Bethesda, MD, 20882, USA</td></tr><tr class="item-phone"><td class="label">Contact Telephone:</td><td class="value">301-443-5061</td></tr><tr class="item-email"><td class="label">Email:</td><td class="value">rasmussenna@mail.nih.gov</td></tr><tr class="item-co-authors"><td class="label">Co-Authors:</td><td class="value">N. Rasmussen, , National Institutes of Health/National Institute of Nursing Research, Bethesda, MD; C. Chaperon, P. Lane, W. Langer, K. Devish, A. Sachs, , University of Nebraska Medical Center, Omaha, NE;</td></tr><tr><td colspan="2" class="item-abstract">Time of day variation in pain sensitivity is evident in practice. This variability may be due to the interaction of temporal and genetic factors. Of particular interest, are genes that are involved in the regulation of biological rhythms. Little is known about the role of circadian locomotor output cycles kaput (Clock) in the time of day variation in pain sensitivity. The objective of this study was to determine the contribution of time of day and genetic or individual variation to the modulation of Clock gene expression in the suprachiasmatic nucleus (SCN) of the brain in response to a painful stimulus. In the study, 8-week old, male, C57BL/6J and DBA/2J mice (n = 22-23 mice/strain) were used. The mice were maintained on a 12:12 light/dark cycle (lights on at 0600). Food and water were available ad libitum. The mice were acclimated to their environment for 1 week prior to exposing the mice to a painful stimulus, a hot-plate. After exposure to a hot-plate at six time points per day including 0000, 0400, 0800, 1200, 1600, and 2000 (n = 3-4 mice/strain/time point), brain tissue was collected for gene expression analysis. Collection of tissue timed for the dark phase (0000, 0400, and 2000) was performed under dim red light. Gene expression analysis was conducted using real-time polymerase chain reaction (RT-PCR). Data were analyzed using ANOVA. Overall, the C57BL/6J mice expressed less Clock in the SCN when compared to the DBA/2J mice. Time of day differences in Clock expression also were noted. Understanding the temporal and genetic variation in the response to pain is important as this knowledge may ultimately lead to the development of assessment and treatment strategies that are most effective and appropriate for each individual at varying times during a 24-hour day.</td></tr></table>en_GB
dc.date.available2011-10-26T21:30:20Z-
dc.date.issued2011-10-17en_GB
dc.date.accessioned2011-10-26T21:30:20Z-
dc.description.sponsorshipMidwest Nursing Research Societyen_GB
All Items in this repository are protected by copyright, with all rights reserved, unless otherwise indicated.