2.50
Hdl Handle:
http://hdl.handle.net/10755/159219
Type:
Presentation
Title:
Challenges in Salivary Cortisol Collection and Analysis for Pediatric Researchers
Abstract:
Challenges in Salivary Cortisol Collection and Analysis for Pediatric Researchers
Conference Sponsor:Midwest Nursing Research Society
Conference Year:2004
Author:Hanrahan, Kirsten, MA, ARNP
P.I. Institution Name:University of Iowa
Title:Project Director
Contact Address:CON-Health Care, 200 Hawkins Drive, 2318 JCP, Iowa City, IA, 52242, USA
Co-Authors:Ann Marie McCarthy, RN, PhD, FAAN, Associate Professor; Charmaine Kleiber, PhD, RN, FAAN
A multisite research project is being conducted to identify factors that predict which children benefit from distraction and which parents are able to successfully coach their children in the use of distraction during a painful procedure. One outcome measure of child distress is salivary cortisol. Potential problems identified included controlling for: collection technique, food and drug influences, diurnal patterns and time of day, and intra and inter-assay reliability. The purpose of this poster is to present procedures for collection and analysis of salivary cortisol in children that assure reliable and valid results. Subjects: When completed, this predictive study will include 540 children, 4 to 10 years of age, from three sites. To date, 80 families have enrolled. Methods: Two specimens are collected on the day of the procedure and two on another, baseline day. A collection procedure was developed that includes chewing sugarless gum and spitting through a straw into a vial. Educational materials are provided to families describing reliable home collection procedures and mailing guidelines. Procedures were developed to control and monitor sample collection, storage, and shipping. Results: Quality control measures were established to ensure the reliability of the assays carried out in the lab. The inter and intra-assay coefficients of variation for the lab are < 12% and 14% in other studies. All samples from a child are sent to the lab in the same batch to eliminate inter-assay variance. Samples in every third batch are assayed in duplicate. A control sample from a collection of pooled sputum is included in each batch of samples sent to the lab. Cortisol levels and quality control data will be presented on the first 60 children. Conclusions: Attention to potential factors influencing salivary cortisol levels in children necessitates the development of collection procedures and analyses to produce reliable and valid measures.
Repository Posting Date:
26-Oct-2011
Date of Publication:
17-Oct-2011
Sponsors:
Midwest Nursing Research Society

Full metadata record

DC FieldValue Language
dc.typePresentationen_GB
dc.titleChallenges in Salivary Cortisol Collection and Analysis for Pediatric Researchersen_GB
dc.identifier.urihttp://hdl.handle.net/10755/159219-
dc.description.abstract<table><tr><td colspan="2" class="item-title">Challenges in Salivary Cortisol Collection and Analysis for Pediatric Researchers </td></tr><tr class="item-sponsor"><td class="label">Conference Sponsor:</td><td class="value">Midwest Nursing Research Society</td></tr><tr class="item-year"><td class="label">Conference Year:</td><td class="value">2004</td></tr><tr class="item-author"><td class="label">Author:</td><td class="value">Hanrahan, Kirsten, MA, ARNP</td></tr><tr class="item-institute"><td class="label">P.I. Institution Name:</td><td class="value">University of Iowa</td></tr><tr class="item-author-title"><td class="label">Title:</td><td class="value">Project Director</td></tr><tr class="item-address"><td class="label">Contact Address:</td><td class="value">CON-Health Care, 200 Hawkins Drive, 2318 JCP, Iowa City, IA, 52242, USA</td></tr><tr class="item-co-authors"><td class="label">Co-Authors:</td><td class="value">Ann Marie McCarthy, RN, PhD, FAAN, Associate Professor; Charmaine Kleiber, PhD, RN, FAAN </td></tr><tr><td colspan="2" class="item-abstract">A multisite research project is being conducted to identify factors that predict which children benefit from distraction and which parents are able to successfully coach their children in the use of distraction during a painful procedure. One outcome measure of child distress is salivary cortisol. Potential problems identified included controlling for: collection technique, food and drug influences, diurnal patterns and time of day, and intra and inter-assay reliability. The purpose of this poster is to present procedures for collection and analysis of salivary cortisol in children that assure reliable and valid results. Subjects: When completed, this predictive study will include 540 children, 4 to 10 years of age, from three sites. To date, 80 families have enrolled. Methods: Two specimens are collected on the day of the procedure and two on another, baseline day. A collection procedure was developed that includes chewing sugarless gum and spitting through a straw into a vial. Educational materials are provided to families describing reliable home collection procedures and mailing guidelines. Procedures were developed to control and monitor sample collection, storage, and shipping. Results: Quality control measures were established to ensure the reliability of the assays carried out in the lab. The inter and intra-assay coefficients of variation for the lab are &lt; 12% and 14% in other studies. All samples from a child are sent to the lab in the same batch to eliminate inter-assay variance. Samples in every third batch are assayed in duplicate. A control sample from a collection of pooled sputum is included in each batch of samples sent to the lab. Cortisol levels and quality control data will be presented on the first 60 children. Conclusions: Attention to potential factors influencing salivary cortisol levels in children necessitates the development of collection procedures and analyses to produce reliable and valid measures. </td></tr></table>en_GB
dc.date.available2011-10-26T21:48:58Z-
dc.date.issued2011-10-17en_GB
dc.date.accessioned2011-10-26T21:48:58Z-
dc.description.sponsorshipMidwest Nursing Research Societyen_GB
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