Effects of Exercise and Environmental Stimulation on Gene Expression in the Adult Rat Brain

2.50
Hdl Handle:
http://hdl.handle.net/10755/159577
Type:
Presentation
Title:
Effects of Exercise and Environmental Stimulation on Gene Expression in the Adult Rat Brain
Abstract:
Effects of Exercise and Environmental Stimulation on Gene Expression in the Adult Rat Brain
Conference Sponsor:Midwest Nursing Research Society
Conference Year:2003
Author:Briones, Tess
Contact Address:Medical-Surgical Nursing, 845 South Damen Avenue, Room 660, M/C 802, Chicago, IL, 60612, USA
Co-Authors:Julie Chai
Brain plasticity is the ability of the central nervous system to alter its structure and function in response to changes in the internal and external environment. Numerous studies have shown that exercise and environmental stimulation in the form of a sensory-rich environment can enhance plasticity in the mature brain. To examine the molecular events induced by exercise and environmental stimulation, we analyzed changes in gene expression in the cortex (both motor and visual cortices), cerebellum, and hippocampus (brain structure involved in learning and memory). Twenty-seven adult male Wistar rats were included in the study and randomly assigned to either exercise, environmental stimulation, or control group. Animals were sacrificed at 1, 3, 7, and 10 days following exercise or environmental stimulation to examine early and late molecular events induced by exercise and environmental stimulation. Ribonucleic acid (RNA) was prepared from the brain regions dissected and double-stranded deoxyribonucleic acid (DNA) was synthesized from the RNA. In vitro transcription using double-stranded complimentary DNA (cDNA) as a template was carried out using gene microarray analysis. cDNA microarray analysis was replicated to determine consistency of results. Analysis of cDNA microarrays is ongoing using the Affymetrix GeneArray Scanner. Findings from this study may be important because characterization of gene expression profiles may shed light into how exercise and/or environmental stimulation can potentially enhance the adaptive plasticity in the adult normal and compromised brain in a manner that may support functional recovery. AN: MN030143
Repository Posting Date:
26-Oct-2011
Date of Publication:
17-Oct-2011
Sponsors:
Midwest Nursing Research Society

Full metadata record

DC FieldValue Language
dc.typePresentationen_GB
dc.titleEffects of Exercise and Environmental Stimulation on Gene Expression in the Adult Rat Brainen_GB
dc.identifier.urihttp://hdl.handle.net/10755/159577-
dc.description.abstract<table><tr><td colspan="2" class="item-title">Effects of Exercise and Environmental Stimulation on Gene Expression in the Adult Rat Brain</td></tr><tr class="item-sponsor"><td class="label">Conference Sponsor:</td><td class="value">Midwest Nursing Research Society</td></tr><tr class="item-year"><td class="label">Conference Year:</td><td class="value">2003</td></tr><tr class="item-author"><td class="label">Author:</td><td class="value">Briones, Tess</td></tr><tr class="item-address"><td class="label">Contact Address:</td><td class="value">Medical-Surgical Nursing, 845 South Damen Avenue, Room 660, M/C 802, Chicago, IL, 60612, USA</td></tr><tr class="item-co-authors"><td class="label">Co-Authors:</td><td class="value">Julie Chai</td></tr><tr><td colspan="2" class="item-abstract">Brain plasticity is the ability of the central nervous system to alter its structure and function in response to changes in the internal and external environment. Numerous studies have shown that exercise and environmental stimulation in the form of a sensory-rich environment can enhance plasticity in the mature brain. To examine the molecular events induced by exercise and environmental stimulation, we analyzed changes in gene expression in the cortex (both motor and visual cortices), cerebellum, and hippocampus (brain structure involved in learning and memory). Twenty-seven adult male Wistar rats were included in the study and randomly assigned to either exercise, environmental stimulation, or control group. Animals were sacrificed at 1, 3, 7, and 10 days following exercise or environmental stimulation to examine early and late molecular events induced by exercise and environmental stimulation. Ribonucleic acid (RNA) was prepared from the brain regions dissected and double-stranded deoxyribonucleic acid (DNA) was synthesized from the RNA. In vitro transcription using double-stranded complimentary DNA (cDNA) as a template was carried out using gene microarray analysis. cDNA microarray analysis was replicated to determine consistency of results. Analysis of cDNA microarrays is ongoing using the Affymetrix GeneArray Scanner. Findings from this study may be important because characterization of gene expression profiles may shed light into how exercise and/or environmental stimulation can potentially enhance the adaptive plasticity in the adult normal and compromised brain in a manner that may support functional recovery. AN: MN030143</td></tr></table>en_GB
dc.date.available2011-10-26T22:08:36Z-
dc.date.issued2011-10-17en_GB
dc.date.accessioned2011-10-26T22:08:36Z-
dc.description.sponsorshipMidwest Nursing Research Societyen_GB
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