Plasma Corticosterone Levels Following Administration of Lipopolysaccharide in Adult and Aged Rats

2.50
Hdl Handle:
http://hdl.handle.net/10755/160363
Type:
Presentation
Title:
Plasma Corticosterone Levels Following Administration of Lipopolysaccharide in Adult and Aged Rats
Abstract:
Plasma Corticosterone Levels Following Administration of Lipopolysaccharide in Adult and Aged Rats
Conference Sponsor:Midwest Nursing Research Society
Conference Year:2005
Author:Kupferschmid, Barbara, PhDc, RN
P.I. Institution Name:University of Michigan
Title:Predoctoral Student
Contact Address:School of Nursing, 2060 SNB 0482, 400 North Ingalls, Ann Arbor, MI, 48109, USA
Contact Telephone:734-769-3311
Co-Authors:Barbara Therrien, PhD, RN, FAAN, Associate Professor
An increase in hypothalamic-pituitary-adrenal axis activity has been
shown to occur after injury, infection, and disease. Lipopolysaccharide
(LPS), an endotoxin, is found in gram-negative bacteria and is often used
in studies to activate the immune response. LPS has become an important
model to study the neuroendocrine responses to bacterial infection. While
some studies have shown that basal corticosterone levels are elevated in
unstressed old animals, there are other contradictory findings. Also,
while corticosterone levels increase in old animals following LPS
administration, some studies have demonstrated that the increase is less
than in young animals. In this study we examined the effect of LPS on
plasma corticosterone levels in adult and old Brown-Norway rats. Adult
(5-6 months) and old (22 months) male rats were randomly assigned to
control or experimental groups. Animals were administered intraperitoneal
100 or 250 & #956; g/kg LPS or saline 4 û 5 hours after lights off. Blood samples
were obtained via the tail vein 1 hour after injections in young animals
and 5 hours after injections in old animals. These times were based on
temperature responses. Blood was collected in tubes containing 40 μl EDTA.
Blood was centrifuged at 3000 rpm for 15 minutes, and plasma frozen and
stored at -80 degrees C. The plasma corticosterone concentrations were determined,
in duplicate, by radioimmunoassay. There was a statistically significant
difference between the control and experimental groups overall (t=-3.974,
df=15.708, p=.001), and between the control and experimental groups in the
old animals (t=-2.494, df=7.307, p=.04) and young animals (t=-3.02,
df=7.332, p=.018) with the corticosterone levels significantly increased
in the experimental groups. There was no difference in corticosterone
increases based on age group. Thus administration of LPS results in an
increase in plasma corticosterone levels and it does not appear to be
influenced by age. Supported by: NIH, NINR, T32 NR07074, Neuroscience
Nursing Foundation.
Repository Posting Date:
26-Oct-2011
Date of Publication:
17-Oct-2011
Sponsors:
Midwest Nursing Research Society

Full metadata record

DC FieldValue Language
dc.typePresentationen_GB
dc.titlePlasma Corticosterone Levels Following Administration of Lipopolysaccharide in Adult and Aged Ratsen_GB
dc.identifier.urihttp://hdl.handle.net/10755/160363-
dc.description.abstract<table><tr><td colspan="2" class="item-title">Plasma Corticosterone Levels Following Administration of Lipopolysaccharide in Adult and Aged Rats</td></tr><tr class="item-sponsor"><td class="label">Conference Sponsor:</td><td class="value">Midwest Nursing Research Society</td></tr><tr class="item-year"><td class="label">Conference Year:</td><td class="value">2005</td></tr><tr class="item-author"><td class="label">Author:</td><td class="value">Kupferschmid, Barbara, PhDc, RN</td></tr><tr class="item-institute"><td class="label">P.I. Institution Name:</td><td class="value">University of Michigan</td></tr><tr class="item-author-title"><td class="label">Title:</td><td class="value">Predoctoral Student</td></tr><tr class="item-address"><td class="label">Contact Address:</td><td class="value">School of Nursing, 2060 SNB 0482, 400 North Ingalls, Ann Arbor, MI, 48109, USA</td></tr><tr class="item-phone"><td class="label">Contact Telephone:</td><td class="value">734-769-3311</td></tr><tr class="item-email"><td class="label">Email:</td><td class="value">bkupf@umich.edu</td></tr><tr class="item-co-authors"><td class="label">Co-Authors:</td><td class="value">Barbara Therrien, PhD, RN, FAAN, Associate Professor</td></tr><tr><td colspan="2" class="item-abstract">An increase in hypothalamic-pituitary-adrenal axis activity has been <br/> shown to occur after injury, infection, and disease. Lipopolysaccharide <br/> (LPS), an endotoxin, is found in gram-negative bacteria and is often used <br/> in studies to activate the immune response. LPS has become an important <br/> model to study the neuroendocrine responses to bacterial infection. While <br/> some studies have shown that basal corticosterone levels are elevated in <br/> unstressed old animals, there are other contradictory findings. Also, <br/> while corticosterone levels increase in old animals following LPS <br/> administration, some studies have demonstrated that the increase is less <br/> than in young animals. In this study we examined the effect of LPS on <br/> plasma corticosterone levels in adult and old Brown-Norway rats. Adult <br/> (5-6 months) and old (22 months) male rats were randomly assigned to <br/> control or experimental groups. Animals were administered intraperitoneal <br/> 100 or 250 &amp; #956; g/kg LPS or saline 4 &ucirc; 5 hours after lights off. Blood samples <br/> were obtained via the tail vein 1 hour after injections in young animals <br/> and 5 hours after injections in old animals. These times were based on <br/> temperature responses. Blood was collected in tubes containing 40 &amp;#956;l EDTA. <br/> Blood was centrifuged at 3000 rpm for 15 minutes, and plasma frozen and <br/> stored at -80 degrees C. The plasma corticosterone concentrations were determined, <br/> in duplicate, by radioimmunoassay. There was a statistically significant <br/> difference between the control and experimental groups overall (t=-3.974, <br/> df=15.708, p=.001), and between the control and experimental groups in the <br/> old animals (t=-2.494, df=7.307, p=.04) and young animals (t=-3.02, <br/> df=7.332, p=.018) with the corticosterone levels significantly increased <br/> in the experimental groups. There was no difference in corticosterone <br/> increases based on age group. Thus administration of LPS results in an <br/> increase in plasma corticosterone levels and it does not appear to be <br/> influenced by age. Supported by: NIH, NINR, T32 NR07074, Neuroscience <br/> Nursing Foundation.</td></tr></table>en_GB
dc.date.available2011-10-26T22:52:20Z-
dc.date.issued2011-10-17en_GB
dc.date.accessioned2011-10-26T22:52:20Z-
dc.description.sponsorshipMidwest Nursing Research Societyen_GB
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