Diaphragm Performance and Fluid Resuscitation Therapies Following Hemorrhagic Shock

2.50
Hdl Handle:
http://hdl.handle.net/10755/160987
Type:
Presentation
Title:
Diaphragm Performance and Fluid Resuscitation Therapies Following Hemorrhagic Shock
Abstract:
Diaphragm Performance and Fluid Resuscitation Therapies Following Hemorrhagic Shock
Conference Sponsor:Midwest Nursing Research Society
Conference Year:2010
Author:Pierce, Janet, DSN, ARNP, CCRN
P.I. Institution Name:Kansas University
Title:School of Nursing
Contact Address:3901 Rainbow Blvd., Kansas City, KS, 66160, USA
Contact Telephone:913-588-1663
Co-Authors:J. Pierce, A. Knight, R. Clancy, School of Nursing, University of Kansas, Kansas City, KS; J. Slusser, Flow Cytometry, University of Kansas, Kansas City, KS;
Purpose: To compare effects of fluid resuscitation and dopamine (DA) on diaphragm shortening (DS), blood flow (DBF), hydrogen peroxide (H2O2) concentration, and apoptosis following hemorrhagic shock (HS). Theoretical Framework: HS results in systemic inflammation, organ injury, and increased reactive oxygen species (ROS). Increased ROS results in decreased diaphragm performance due to lipid peroxidation, protein degradation, and apoptosis. Methods: DS and DBF were measured at baseline, shock, and treatment. HS was elicited by removing 40% of blood over 60 minutes. During the next 30 minutes, lactated Ringer's (LR), LR plus DA, Hespan, or Hespan plus DA were infused. Diaphragm H2O2 and apoptosis were measured using laser scanning cytometry and fluorescent microscopy. Results: At baseline, DS (mm) was 0.61 and 0.86 at HS. After infusing LR, DS was 0.60, 0.92 for LR plus DA, 0.89 for Hespan, and 0.97 for Hespan plus DA. At baseline DBF (mL/min/gm) was 0.75 and 0.57 at HS. DBF was 0.46 for LR, 0.79 for LR plus DA, 0.95 for Hespan, and 1.2 for Hespan plus DA. H2O2 (fluorescence) with LR = 8.4 x106, LR plus DA = 2.0 x 106, Hespan = 5.4 x 106, and Hespan plus DA = 1.7 x 106. Percent apoptosis was 34.5 for LR, 1.2 for LR plus DA, 11.7 for Hespan, and 6.8 for Hespan plus DA. Conclusions: LR failed to maintain DS equal to that at HS. The alterations in DS were directly proportional to DBF changes. LR plus DA, Hespan, and Hespan plus DA resulted in H2O2 being significantly less than LR alone. Apoptosis results were proportional to H2O2. This suggests that these fluids are more effective than LR alone in decreasing diaphragm ROS and apoptosis. Funding Source: TriService Nursing Research Program, #HU0001-05-1-TS11
Repository Posting Date:
26-Oct-2011
Date of Publication:
17-Oct-2011
Sponsors:
Midwest Nursing Research Society

Full metadata record

DC FieldValue Language
dc.typePresentationen_GB
dc.titleDiaphragm Performance and Fluid Resuscitation Therapies Following Hemorrhagic Shocken_GB
dc.identifier.urihttp://hdl.handle.net/10755/160987-
dc.description.abstract<table><tr><td colspan="2" class="item-title">Diaphragm Performance and Fluid Resuscitation Therapies Following Hemorrhagic Shock</td></tr><tr class="item-sponsor"><td class="label">Conference Sponsor:</td><td class="value">Midwest Nursing Research Society</td></tr><tr class="item-year"><td class="label">Conference Year:</td><td class="value">2010</td></tr><tr class="item-author"><td class="label">Author:</td><td class="value">Pierce, Janet, DSN, ARNP, CCRN</td></tr><tr class="item-institute"><td class="label">P.I. Institution Name:</td><td class="value">Kansas University</td></tr><tr class="item-author-title"><td class="label">Title:</td><td class="value">School of Nursing</td></tr><tr class="item-address"><td class="label">Contact Address:</td><td class="value">3901 Rainbow Blvd., Kansas City, KS, 66160, USA</td></tr><tr class="item-phone"><td class="label">Contact Telephone:</td><td class="value">913-588-1663</td></tr><tr class="item-email"><td class="label">Email:</td><td class="value">jpierce@kumc.edu</td></tr><tr class="item-co-authors"><td class="label">Co-Authors:</td><td class="value">J. Pierce, A. Knight, R. Clancy, School of Nursing, University of Kansas, Kansas City, KS; J. Slusser, Flow Cytometry, University of Kansas, Kansas City, KS;</td></tr><tr><td colspan="2" class="item-abstract">Purpose: To compare effects of fluid resuscitation and dopamine (DA) on diaphragm shortening (DS), blood flow (DBF), hydrogen peroxide (H2O2) concentration, and apoptosis following hemorrhagic shock (HS). Theoretical Framework: HS results in systemic inflammation, organ injury, and increased reactive oxygen species (ROS). Increased ROS results in decreased diaphragm performance due to lipid peroxidation, protein degradation, and apoptosis. Methods: DS and DBF were measured at baseline, shock, and treatment. HS was elicited by removing 40% of blood over 60 minutes. During the next 30 minutes, lactated Ringer's (LR), LR plus DA, Hespan, or Hespan plus DA were infused. Diaphragm H2O2 and apoptosis were measured using laser scanning cytometry and fluorescent microscopy. Results: At baseline, DS (mm) was 0.61 and 0.86 at HS. After infusing LR, DS was 0.60, 0.92 for LR plus DA, 0.89 for Hespan, and 0.97 for Hespan plus DA. At baseline DBF (mL/min/gm) was 0.75 and 0.57 at HS. DBF was 0.46 for LR, 0.79 for LR plus DA, 0.95 for Hespan, and 1.2 for Hespan plus DA. H2O2 (fluorescence) with LR = 8.4 x106, LR plus DA = 2.0 x 106, Hespan = 5.4 x 106, and Hespan plus DA = 1.7 x 106. Percent apoptosis was 34.5 for LR, 1.2 for LR plus DA, 11.7 for Hespan, and 6.8 for Hespan plus DA. Conclusions: LR failed to maintain DS equal to that at HS. The alterations in DS were directly proportional to DBF changes. LR plus DA, Hespan, and Hespan plus DA resulted in H2O2 being significantly less than LR alone. Apoptosis results were proportional to H2O2. This suggests that these fluids are more effective than LR alone in decreasing diaphragm ROS and apoptosis. Funding Source: TriService Nursing Research Program, #HU0001-05-1-TS11</td></tr></table>en_GB
dc.date.available2011-10-26T23:14:03Z-
dc.date.issued2011-10-17en_GB
dc.date.accessioned2011-10-26T23:14:03Z-
dc.description.sponsorshipMidwest Nursing Research Societyen_GB
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