Lateral Hypothalamus Modifies Pain Through a Substance P Connection with the Periaqueductal Gray

2.50
Hdl Handle:
http://hdl.handle.net/10755/161211
Type:
Presentation
Title:
Lateral Hypothalamus Modifies Pain Through a Substance P Connection with the Periaqueductal Gray
Abstract:
Lateral Hypothalamus Modifies Pain Through a Substance P Connection with the Periaqueductal Gray
Conference Sponsor:Midwest Nursing Research Society
Conference Year:2005
Author:Pizzi, Julie, MS, BSN, RN
P.I. Institution Name:University of Illinois at Chicago
Title:Predoctoral Student
Contact Address:Medical-Surgical Nursing, 801 S. Wells #1005, Chicago, IL, 60607, USA
Contact Telephone:312-996-3935
Co-Authors:Janean E. Holden, PhD, Associate Professor
Pain is a complex phenomenon and not completely understood.
Understanding how the body modulates pain may lead to better care for
patients in pain. Stimulation of the lateral hypothalamus (LH) produces
analgesia in part through connections with the ventrolateral
periaqueductal gray (vPAG), an area in the brainstem known to produce
endogenous analgesia. However, the specific neurotransmitters involved
have not been clearly defined. Substance P (subP) containing neurons have
been identified in the LH and subP receptors have been identified on
neurons in the vPAG. To test the hypothesis that LH-induced analgesia is
mediated in part by a subP connection with the vPAG, the cholinergic
agonist carbachol (125 nmol) was microinjected into the left LH of lightly
anesthetized female Sprague-Dawley rats (250-350g), and moderate analgesia
was obtained on the tail flick test . Cobalt Chloride (23 micrograms/0.5 microliters), which
blocks all synaptic activity in the area of injection, was microinjected
into the left vPAG and partially blocked analgesia compared to controls
(3.11 plus or minus 0.18 vs. 4.96 plus or minus 0.14 sec., respectively, p<0.05). The subP
receptor antagonist L-703,606 (2 micrograms/5 microliters) was then microinjected into the
left PAG and also partially blocked LH-induced analgesia compared to
controls (3.12 plus or minus 0.16 vs. 4.96 plus or minus 0.14 sec., respectively, p<0.05). Neither
cobalt chloride nor L-703,606 produced a significant effect when
microinjected into the vPAG alone. These data suggest that analgesia from
LH stimulation is partially mediated subP connections with the vPAG, but
this connection is not tonically active. Support Contributed By: USPHS
grant NR04778 and T32-NR07075 from the National Institute of Nursing
Research, and a predoctoral fellowship from the National Science Foundation.
Repository Posting Date:
26-Oct-2011
Date of Publication:
17-Oct-2011
Sponsors:
Midwest Nursing Research Society

Full metadata record

DC FieldValue Language
dc.typePresentationen_GB
dc.titleLateral Hypothalamus Modifies Pain Through a Substance P Connection with the Periaqueductal Grayen_GB
dc.identifier.urihttp://hdl.handle.net/10755/161211-
dc.description.abstract<table><tr><td colspan="2" class="item-title">Lateral Hypothalamus Modifies Pain Through a Substance P Connection with the Periaqueductal Gray</td></tr><tr class="item-sponsor"><td class="label">Conference Sponsor:</td><td class="value">Midwest Nursing Research Society</td></tr><tr class="item-year"><td class="label">Conference Year:</td><td class="value">2005</td></tr><tr class="item-author"><td class="label">Author:</td><td class="value">Pizzi, Julie, MS, BSN, RN</td></tr><tr class="item-institute"><td class="label">P.I. Institution Name:</td><td class="value">University of Illinois at Chicago</td></tr><tr class="item-author-title"><td class="label">Title:</td><td class="value">Predoctoral Student</td></tr><tr class="item-address"><td class="label">Contact Address:</td><td class="value">Medical-Surgical Nursing, 801 S. Wells #1005, Chicago, IL, 60607, USA</td></tr><tr class="item-phone"><td class="label">Contact Telephone:</td><td class="value">312-996-3935</td></tr><tr class="item-email"><td class="label">Email:</td><td class="value">jpizzi1@uic.edu</td></tr><tr class="item-co-authors"><td class="label">Co-Authors:</td><td class="value">Janean E. Holden, PhD, Associate Professor</td></tr><tr><td colspan="2" class="item-abstract">Pain is a complex phenomenon and not completely understood. <br/> Understanding how the body modulates pain may lead to better care for <br/> patients in pain. Stimulation of the lateral hypothalamus (LH) produces <br/> analgesia in part through connections with the ventrolateral <br/> periaqueductal gray (vPAG), an area in the brainstem known to produce <br/> endogenous analgesia. However, the specific neurotransmitters involved <br/> have not been clearly defined. Substance P (subP) containing neurons have <br/> been identified in the LH and subP receptors have been identified on <br/> neurons in the vPAG. To test the hypothesis that LH-induced analgesia is <br/> mediated in part by a subP connection with the vPAG, the cholinergic <br/> agonist carbachol (125 nmol) was microinjected into the left LH of lightly <br/> anesthetized female Sprague-Dawley rats (250-350g), and moderate analgesia <br/> was obtained on the tail flick test . Cobalt Chloride (23 micrograms/0.5 microliters), which <br/> blocks all synaptic activity in the area of injection, was microinjected <br/> into the left vPAG and partially blocked analgesia compared to controls <br/> (3.11 plus or minus 0.18 vs. 4.96 plus or minus 0.14 sec., respectively, p&lt;0.05). The subP <br/> receptor antagonist L-703,606 (2 micrograms/5 microliters) was then microinjected into the <br/> left PAG and also partially blocked LH-induced analgesia compared to <br/> controls (3.12 plus or minus 0.16 vs. 4.96 plus or minus 0.14 sec., respectively, p&lt;0.05). Neither <br/> cobalt chloride nor L-703,606 produced a significant effect when <br/> microinjected into the vPAG alone. These data suggest that analgesia from <br/> LH stimulation is partially mediated subP connections with the vPAG, but <br/> this connection is not tonically active. Support Contributed By: USPHS <br/> grant NR04778 and T32-NR07075 from the National Institute of Nursing <br/> Research, and a predoctoral fellowship from the National Science Foundation.</td></tr></table>en_GB
dc.date.available2011-10-26T23:17:42Z-
dc.date.issued2011-10-17en_GB
dc.date.accessioned2011-10-26T23:17:42Z-
dc.description.sponsorshipMidwest Nursing Research Societyen_GB
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