Changes in Synaptic Mitochondria After Cerebral Ischemia and Behavioral Intervention

2.50
Hdl Handle:
http://hdl.handle.net/10755/161287
Type:
Presentation
Title:
Changes in Synaptic Mitochondria After Cerebral Ischemia and Behavioral Intervention
Abstract:
Changes in Synaptic Mitochondria After Cerebral Ischemia and Behavioral Intervention
Conference Sponsor:Midwest Nursing Research Society
Conference Year:2004
Author:Briones, Tess, PhD, RN
Title:Assistant Professor
Contact Address:, 845 S. Damen Ave.- Rm 660, M/C 802, Chicago, IL, 60612, USA
There is now increasing evidence that behavioral interventions in the form of exercise (EX) and exposure to sensory-rich environment (EC) after cerebral ischemia can result in increased ratio of synapses to neuron (synaptogenesis). However, the metabolic correlates of these synaptic changes remain unclear. Since the mitochondria play an important role in CNS metabolism, in this study we quantified the number and size of mitochondria at synaptic terminals located in the hippocampus (brain region involved in memory processing and also the area that is most vulnerable to ischemic damage). Forty-two adult male Wistar rats were included in the study and randomly assigned to either the ischemic or sham group. Following ischemic or sham surgery (experimental condition), rats were further randomized to either one of 3 behavioral interventions: EC, EX, or social condition (paired housing - control). Behavioral intervention was carried out for 14 days then the brains were processed for electron microscopy. Two sets of eight electron micrographs were taken systematically from the serial sections obtained from the hippocampus and data were analyzed using the physical disector method (involves comparing adjacent sections and thereby approximates "reconstruction"). Results showed that ischemic animals have significantly increased number of synaptic mitochondria per neuron compared to shams. Furthermore, animals in EC and EX have significantly more synaptic mitochondria compared to their SC counterparts. Interestingly, mitochondrial size was not affected by either experimental condition or behavioral intervention. Because metabolic capacity is affected by the number of mitochondria, these results suggest the possibility of heightened synaptic activity which is likely to be causally linked to synaptogenesis. Supported by NIH NR05260
Repository Posting Date:
26-Oct-2011
Date of Publication:
17-Oct-2011
Sponsors:
Midwest Nursing Research Society

Full metadata record

DC FieldValue Language
dc.typePresentationen_GB
dc.titleChanges in Synaptic Mitochondria After Cerebral Ischemia and Behavioral Interventionen_GB
dc.identifier.urihttp://hdl.handle.net/10755/161287-
dc.description.abstract<table><tr><td colspan="2" class="item-title">Changes in Synaptic Mitochondria After Cerebral Ischemia and Behavioral Intervention </td></tr><tr class="item-sponsor"><td class="label">Conference Sponsor:</td><td class="value">Midwest Nursing Research Society</td></tr><tr class="item-year"><td class="label">Conference Year:</td><td class="value">2004</td></tr><tr class="item-author"><td class="label">Author:</td><td class="value">Briones, Tess, PhD, RN</td></tr><tr class="item-author-title"><td class="label">Title:</td><td class="value">Assistant Professor</td></tr><tr class="item-address"><td class="label">Contact Address:</td><td class="value">, 845 S. Damen Ave.- Rm 660, M/C 802, Chicago, IL, 60612, USA</td></tr><tr><td colspan="2" class="item-abstract">There is now increasing evidence that behavioral interventions in the form of exercise (EX) and exposure to sensory-rich environment (EC) after cerebral ischemia can result in increased ratio of synapses to neuron (synaptogenesis). However, the metabolic correlates of these synaptic changes remain unclear. Since the mitochondria play an important role in CNS metabolism, in this study we quantified the number and size of mitochondria at synaptic terminals located in the hippocampus (brain region involved in memory processing and also the area that is most vulnerable to ischemic damage). Forty-two adult male Wistar rats were included in the study and randomly assigned to either the ischemic or sham group. Following ischemic or sham surgery (experimental condition), rats were further randomized to either one of 3 behavioral interventions: EC, EX, or social condition (paired housing - control). Behavioral intervention was carried out for 14 days then the brains were processed for electron microscopy. Two sets of eight electron micrographs were taken systematically from the serial sections obtained from the hippocampus and data were analyzed using the physical disector method (involves comparing adjacent sections and thereby approximates &quot;reconstruction&quot;). Results showed that ischemic animals have significantly increased number of synaptic mitochondria per neuron compared to shams. Furthermore, animals in EC and EX have significantly more synaptic mitochondria compared to their SC counterparts. Interestingly, mitochondrial size was not affected by either experimental condition or behavioral intervention. Because metabolic capacity is affected by the number of mitochondria, these results suggest the possibility of heightened synaptic activity which is likely to be causally linked to synaptogenesis. Supported by NIH NR05260 </td></tr></table>en_GB
dc.date.available2011-10-26T23:18:55Z-
dc.date.issued2011-10-17en_GB
dc.date.accessioned2011-10-26T23:18:55Z-
dc.description.sponsorshipMidwest Nursing Research Societyen_GB
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