Cytoskeletal Changes in Endothelial Cells after Treatment with 5-Ht and 5-Ht Inhibitors

2.50
Hdl Handle:
http://hdl.handle.net/10755/161636
Type:
Presentation
Title:
Cytoskeletal Changes in Endothelial Cells after Treatment with 5-Ht and 5-Ht Inhibitors
Abstract:
Cytoskeletal Changes in Endothelial Cells after Treatment with 5-Ht and 5-Ht Inhibitors
Conference Sponsor:Midwest Nursing Research Society
Conference Year:2002
Author:Gordon, Joanne, PhD
P.I. Institution Name:Southwest Missouri State University
Title:Professor
Contact Address:Department of Nursing, 335 Professional Building, 901 South National Avenue, Springfield, MO, 65804, USA
Contact Telephone:417.836.7601
Inflammation leads to increased vascular permeability and loss of fluid from the intravascular space to the interstitial space. This loss of fluid can affect the blood volume and blood pressure of individuals with acute systemic inflammatory responses. This study explores the effect of one inflammatory chemical, 5-hydroxytryptamine (5-HT, serotonin) on the cytoskeleton of calf pulmonary artery endothelial cells (EC).Besides causing cell retraction and an increase in intercellular gaps that can lead to extravasation, 5-HT has been found to affect the size of intracellular pores. Actin and myosin filaments are associated with these endothelial intracellular pores. Actin also contributes to the structure of the EC cytoskeleton and is found as G-actin, a globular monomer, and F-actin, a filamentous polymer. To explore the effect of 5-HT on actin filaments and intracellular pores, ECs were grown on glass slides; treated with 1 mM 5-HT; labeled with rhodamine phalloidin, a specific F-actin filament fluorochrome; and observed utilizing an epifluorescent microscope. Images were captured with a photomicrographic system and analyzed for actin morphology. Control slides showed the expected EC cell structure, with broad membrane protrusions (lamellipodia) at the forward moving end of the cell, finger-like structures on the cell periphery (filopodia), and ruffles, where the membrane lifts off of the substrate. Actin filaments appeared as networks and bundles, with loosely packed crisscrossed filaments. Cells treated with 5-HT showed fewer actin filaments in the cell periphery and smoother, denser edges, suggestive of decreased adherence to the substrate and cell contraction. Cells pre-treated with a 5-HT receptor inhibitor prior to 5-HT application showed morphology and actin distribution similar to the control cells. This research suggests that 5-HT may have an effect on vascular permeability and extravasation at the EC level. Blocking of 5-HT receptors with a receptor-inhibitor was shown to prevent the effects of 5-HT.
Repository Posting Date:
26-Oct-2011
Date of Publication:
17-Oct-2011
Sponsors:
Midwest Nursing Research Society

Full metadata record

DC FieldValue Language
dc.typePresentationen_GB
dc.titleCytoskeletal Changes in Endothelial Cells after Treatment with 5-Ht and 5-Ht Inhibitorsen_GB
dc.identifier.urihttp://hdl.handle.net/10755/161636-
dc.description.abstract<table><tr><td colspan="2" class="item-title">Cytoskeletal Changes in Endothelial Cells after Treatment with 5-Ht and 5-Ht Inhibitors</td></tr><tr class="item-sponsor"><td class="label">Conference Sponsor:</td><td class="value">Midwest Nursing Research Society</td></tr><tr class="item-year"><td class="label">Conference Year:</td><td class="value">2002</td></tr><tr class="item-author"><td class="label">Author:</td><td class="value">Gordon, Joanne, PhD</td></tr><tr class="item-institute"><td class="label">P.I. Institution Name:</td><td class="value">Southwest Missouri State University</td></tr><tr class="item-author-title"><td class="label">Title:</td><td class="value">Professor</td></tr><tr class="item-address"><td class="label">Contact Address:</td><td class="value">Department of Nursing, 335 Professional Building, 901 South National Avenue, Springfield, MO, 65804, USA</td></tr><tr class="item-phone"><td class="label">Contact Telephone:</td><td class="value">417.836.7601</td></tr><tr class="item-email"><td class="label">Email:</td><td class="value">jmg230f@smsu.edu</td></tr><tr><td colspan="2" class="item-abstract">Inflammation leads to increased vascular permeability and loss of fluid from the intravascular space to the interstitial space. This loss of fluid can affect the blood volume and blood pressure of individuals with acute systemic inflammatory responses. This study explores the effect of one inflammatory chemical, 5-hydroxytryptamine (5-HT, serotonin) on the cytoskeleton of calf pulmonary artery endothelial cells (EC).Besides causing cell retraction and an increase in intercellular gaps that can lead to extravasation, 5-HT has been found to affect the size of intracellular pores. Actin and myosin filaments are associated with these endothelial intracellular pores. Actin also contributes to the structure of the EC cytoskeleton and is found as G-actin, a globular monomer, and F-actin, a filamentous polymer. To explore the effect of 5-HT on actin filaments and intracellular pores, ECs were grown on glass slides; treated with 1 mM 5-HT; labeled with rhodamine phalloidin, a specific F-actin filament fluorochrome; and observed utilizing an epifluorescent microscope. Images were captured with a photomicrographic system and analyzed for actin morphology. Control slides showed the expected EC cell structure, with broad membrane protrusions (lamellipodia) at the forward moving end of the cell, finger-like structures on the cell periphery (filopodia), and ruffles, where the membrane lifts off of the substrate. Actin filaments appeared as networks and bundles, with loosely packed crisscrossed filaments. Cells treated with 5-HT showed fewer actin filaments in the cell periphery and smoother, denser edges, suggestive of decreased adherence to the substrate and cell contraction. Cells pre-treated with a 5-HT receptor inhibitor prior to 5-HT application showed morphology and actin distribution similar to the control cells. This research suggests that 5-HT may have an effect on vascular permeability and extravasation at the EC level. Blocking of 5-HT receptors with a receptor-inhibitor was shown to prevent the effects of 5-HT.</td></tr></table>en_GB
dc.date.available2011-10-26T23:24:40Z-
dc.date.issued2011-10-17en_GB
dc.date.accessioned2011-10-26T23:24:40Z-
dc.description.sponsorshipMidwest Nursing Research Societyen_GB
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