2.50
Hdl Handle:
http://hdl.handle.net/10755/163265
Category:
Abstract
Type:
Presentation
Title:
NIH Collaborative Intramural Mitochondrial Research Project
Author(s):
Voss, Joachim G.; Morse, Caryn; Kovacs, Joseph A.; Dalakas, Marinos C.; Danner, Robert; Munson, Peter; Fischer, Steven
Author Details:
Joachim G. Voss, PhD, RN, Research Fellow, National Institutes of Health Neuromuscular Diseases Section, Bethesda, Maryland, USA, email: vossj@ninds.nih.gov; Caryn Morse, MD, MPH; Joseph A. Kovacs, MD; Marinos C. Dalakas, MD; Robert Danner, MD; Peter Munson, PhD; Steven Fischer, MD
Abstract:
Purpose: The etiology of fatigue in HIV/AIDS is multifactorial. While measures have been developed to capture subjective symptom experiences of fatigue, no objective biomarkers have been established to quantify the presence of this disabling symptom. Therefore, a research collaboration was established between four intramural Institutes at NIH to investigate how the HIV virus infection and antiretroviral treatments effect mitochondrial function and fatigue. Theoretical Framework: As theoretical framework, we combined the major concepts of the UCSF Symptom Management Model of symptom perception, management and outcome, embedded in the person, environment, health and illness concepts, with the biological model of energy production and mitochondrial function. Methods (Design, Sample, Setting, Measures, Analysis): The underlying assumption is that if the HIV infection and/or antiretroviral treatment cause mitochondrial dysfunction, both can cause fatigue through a reduction in energy output. To validate these assumptions a clinical research protocol was developed. For the protocol, patients complete questionnaires related to fatigue and undergo muscle and fat biopsy and several blood draws. A custom Microarray chip was designed for gene expression studies, as well as a mitochondrial DNA assay to quantify DNA depletion. Additionally, muscle biopsies are evaluated for biochemical and cytoarchitecture changes. Results: *We have currently enrolled 14 subjects and 8 subjects have completed the study; data collection is ongoing until 2006. The custom mitochondrial Microarray chip has been pilot-tested against the current gold standard and reliability was established at a correlation coefficient of r=0.98. Conclusions and Implications: Through collaborative research the causes of fatigue in HIV/AIDS may be faster characterized and the chance of discovery of an objective biomarker for fatigue largely increased.
Repository Posting Date:
27-Oct-2011
Date of Publication:
27-Oct-2011
Conference Date:
2006
Conference Name:
18th Annual Scientific Sessions
Conference Host:
Eastern Nursing Research Society
Conference Location:
Cherry Hill, New Jersey
Description:
�New Momentum for Nursing Research: Multidisciplinary Alliances�, held on April 20th -22nd at the Hilton in Cherry Hill, New Jersey
Note:
This is an abstract-only submission. If the author has submitted a full-text item based on this abstract, you may find it by browsing the Virginia Henderson Global Nursing e-Repository by author. If author contact information is available in this abstract, please feel free to contact him or her with your queries regarding this submission. Alternatively, please contact the conference host, journal, or publisher (according to the circumstance) for further details regarding this item. If a citation is listed in this record, the item has been published and is available via open-access avenues or a journal/database subscription. Contact your library for assistance in obtaining the as-published article.

Full metadata record

DC FieldValue Language
dc.type.categoryAbstracten_US
dc.typePresentationen_GB
dc.titleNIH Collaborative Intramural Mitochondrial Research Projecten_GB
dc.contributor.authorVoss, Joachim G.en_US
dc.contributor.authorMorse, Carynen_US
dc.contributor.authorKovacs, Joseph A.en_US
dc.contributor.authorDalakas, Marinos C.en_US
dc.contributor.authorDanner, Roberten_US
dc.contributor.authorMunson, Peteren_US
dc.contributor.authorFischer, Stevenen_US
dc.author.detailsJoachim G. Voss, PhD, RN, Research Fellow, National Institutes of Health Neuromuscular Diseases Section, Bethesda, Maryland, USA, email: vossj@ninds.nih.gov; Caryn Morse, MD, MPH; Joseph A. Kovacs, MD; Marinos C. Dalakas, MD; Robert Danner, MD; Peter Munson, PhD; Steven Fischer, MDen_US
dc.identifier.urihttp://hdl.handle.net/10755/163265-
dc.description.abstractPurpose: The etiology of fatigue in HIV/AIDS is multifactorial. While measures have been developed to capture subjective symptom experiences of fatigue, no objective biomarkers have been established to quantify the presence of this disabling symptom. Therefore, a research collaboration was established between four intramural Institutes at NIH to investigate how the HIV virus infection and antiretroviral treatments effect mitochondrial function and fatigue. Theoretical Framework: As theoretical framework, we combined the major concepts of the UCSF Symptom Management Model of symptom perception, management and outcome, embedded in the person, environment, health and illness concepts, with the biological model of energy production and mitochondrial function. Methods (Design, Sample, Setting, Measures, Analysis): The underlying assumption is that if the HIV infection and/or antiretroviral treatment cause mitochondrial dysfunction, both can cause fatigue through a reduction in energy output. To validate these assumptions a clinical research protocol was developed. For the protocol, patients complete questionnaires related to fatigue and undergo muscle and fat biopsy and several blood draws. A custom Microarray chip was designed for gene expression studies, as well as a mitochondrial DNA assay to quantify DNA depletion. Additionally, muscle biopsies are evaluated for biochemical and cytoarchitecture changes. Results: *We have currently enrolled 14 subjects and 8 subjects have completed the study; data collection is ongoing until 2006. The custom mitochondrial Microarray chip has been pilot-tested against the current gold standard and reliability was established at a correlation coefficient of r=0.98. Conclusions and Implications: Through collaborative research the causes of fatigue in HIV/AIDS may be faster characterized and the chance of discovery of an objective biomarker for fatigue largely increased.en_GB
dc.date.available2011-10-27T11:04:22Z-
dc.date.issued2011-10-27en_GB
dc.date.accessioned2011-10-27T11:04:22Z-
dc.conference.date2006en_US
dc.conference.name18th Annual Scientific Sessionsen_US
dc.conference.hostEastern Nursing Research Societyen_US
dc.conference.locationCherry Hill, New Jerseyen_US
dc.description�New Momentum for Nursing Research: Multidisciplinary Alliances�, held on April 20th -22nd at the Hilton in Cherry Hill, New Jerseyen_US
dc.description.noteThis is an abstract-only submission. If the author has submitted a full-text item based on this abstract, you may find it by browsing the Virginia Henderson Global Nursing e-Repository by author. If author contact information is available in this abstract, please feel free to contact him or her with your queries regarding this submission. Alternatively, please contact the conference host, journal, or publisher (according to the circumstance) for further details regarding this item. If a citation is listed in this record, the item has been published and is available via open-access avenues or a journal/database subscription. Contact your library for assistance in obtaining the as-published article.-
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