2.50
Hdl Handle:
http://hdl.handle.net/10755/163602
Category:
Abstract
Type:
Presentation
Title:
Minimally invasive techniques to monitor immune system activation
Author(s):
Corwin, Elizabeth; Johnston, Nancy A.
Author Details:
Elizabeth Corwin, Emory University, Atlanta, Georgia, USA, email: ejcorwi@emory.edu; Nancy A. Johnston
Abstract:
Purpose: Clinical research studies frequently require multiple blood samplings to gain insight into changes in health status over time. The literature suggests, however, that subjects frequently decline to participate in studies requiring serial blood sampling, or discontinue participation because of adverse reactions to blood draws. In our laboratory, we are interested in following immune responsiveness of pregnant women at risk of preterm labor from week 26 of gestation until delivery. Specific Aims: In order to minimize discomfort and maximize recruitment of subjects, however, before beginning this study, we undertook a bench study to validate the use of minimally invasive techniques to obtain fluid for hormonal and immune assays. Methods: Twenty non-pregnant control subjects provided a clean-catch urine sample, a saliva sample, a finger-prick blood sample, and a venous blood sample via venipuncture. Nurse researchers processed and later analyzed all samples by enzyme-linked immunosorbent assay (ELISA) for interleukin-1, interleukin-6, tumor necrosis factor, Creactive protein, and cortisol. Results and conclusions: Endocrine and immune peptide levels were compared between sampling sources (i.e., blood, saliva, and urine). Results identified which sampling procedures were comparable and valid for use in non-invasive clinical studies. Implications: Minimally invasive sampling exists as a valid technique that will allow for long-term protocols in a variety of research studies of interest to nurses. Data collected from this bench pilot project will be the basis of a doctoral study to identify immune and hormonal status in women at risk of preterm labor.
Repository Posting Date:
27-Oct-2011
Date of Publication:
27-Oct-2011
Conference Date:
2002
Conference Name:
14th Annual Scientific Sessions
Conference Host:
Eastern Nursing Research Society
Conference Location:
University Park, Pennsylvania, USA
Note:
This is an abstract-only submission. If the author has submitted a full-text item based on this abstract, you may find it by browsing the Virginia Henderson Global Nursing e-Repository by author. If author contact information is available in this abstract, please feel free to contact him or her with your queries regarding this submission. Alternatively, please contact the conference host, journal, or publisher (according to the circumstance) for further details regarding this item. If a citation is listed in this record, the item has been published and is available via open-access avenues or a journal/database subscription. Contact your library for assistance in obtaining the as-published article.

Full metadata record

DC FieldValue Language
dc.type.categoryAbstracten_US
dc.typePresentationen_GB
dc.titleMinimally invasive techniques to monitor immune system activationen_GB
dc.contributor.authorCorwin, Elizabethen_US
dc.contributor.authorJohnston, Nancy A.en_US
dc.author.detailsElizabeth Corwin, Emory University, Atlanta, Georgia, USA, email: ejcorwi@emory.edu; Nancy A. Johnstonen_US
dc.identifier.urihttp://hdl.handle.net/10755/163602-
dc.description.abstractPurpose: Clinical research studies frequently require multiple blood samplings to gain insight into changes in health status over time. The literature suggests, however, that subjects frequently decline to participate in studies requiring serial blood sampling, or discontinue participation because of adverse reactions to blood draws. In our laboratory, we are interested in following immune responsiveness of pregnant women at risk of preterm labor from week 26 of gestation until delivery. Specific Aims: In order to minimize discomfort and maximize recruitment of subjects, however, before beginning this study, we undertook a bench study to validate the use of minimally invasive techniques to obtain fluid for hormonal and immune assays. Methods: Twenty non-pregnant control subjects provided a clean-catch urine sample, a saliva sample, a finger-prick blood sample, and a venous blood sample via venipuncture. Nurse researchers processed and later analyzed all samples by enzyme-linked immunosorbent assay (ELISA) for interleukin-1, interleukin-6, tumor necrosis factor, Creactive protein, and cortisol. Results and conclusions: Endocrine and immune peptide levels were compared between sampling sources (i.e., blood, saliva, and urine). Results identified which sampling procedures were comparable and valid for use in non-invasive clinical studies. Implications: Minimally invasive sampling exists as a valid technique that will allow for long-term protocols in a variety of research studies of interest to nurses. Data collected from this bench pilot project will be the basis of a doctoral study to identify immune and hormonal status in women at risk of preterm labor.en_GB
dc.date.available2011-10-27T11:10:27Z-
dc.date.issued2011-10-27en_GB
dc.date.accessioned2011-10-27T11:10:27Z-
dc.conference.date2002en_US
dc.conference.name14th Annual Scientific Sessionsen_US
dc.conference.hostEastern Nursing Research Societyen_US
dc.conference.locationUniversity Park, Pennsylvania, USAen_US
dc.description.noteThis is an abstract-only submission. If the author has submitted a full-text item based on this abstract, you may find it by browsing the Virginia Henderson Global Nursing e-Repository by author. If author contact information is available in this abstract, please feel free to contact him or her with your queries regarding this submission. Alternatively, please contact the conference host, journal, or publisher (according to the circumstance) for further details regarding this item. If a citation is listed in this record, the item has been published and is available via open-access avenues or a journal/database subscription. Contact your library for assistance in obtaining the as-published article.-
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