2.50
Hdl Handle:
http://hdl.handle.net/10755/175488
Type:
Research Study
Title:
Stability and bactericidal activity of chlorine solutions
Abstract:
Stability and bactericidal activity of chlorine solutions
OBJECTIVES: To determine the stability of sodium hypochlorite (diluted household bleach) when stored for 30 days in various types of containers and to determine the efficacy of low concentrations of free available chlorine to inactivate test bacteria. DESIGN: Laboratory-based study. Solutions of standard household bleach were prepared using tap water or sterile distilled water at dilutions of 1:100, 1:50, and 1:5. Chlorine concentrations were measured, and then the solutions were placed into five polyethylene containers and left at room temperature (20 degrees C) under various conditions (translucent containers with light exposure and with or without air; brown opaque container without light or air exposure). Samples for chlorine and pH determinations were taken at time 0 and on days 7, 14, 21, 30, and 40. Bactericidal activity of chlorine solutions was assessed using the Association of Official Analytical Chemists Use-Dilution Method. Test bacteria included Pseudomonas aeruginosa, Staphylococcus aureus, and Salmonella choleraesuis. RESULTS: Chlorine concentrations at 30 days varied from the 40% to 50% range for 1:50 or 1:100 dilutions stored in containers other than closed brown containers to 83% to 85% for the 1:5 dilution stored in closed but non-opaque containers to 97% to 100% for 1:50 or 1:5 solutions stored in closed brown containers. The lowest concentration of sodium hypochlorite solution that reliably inactivated all the test organisms was 100 ppm. CONCLUSIONS: These data suggest that chlorine solutions do not need to be prepared fresh daily, as is recommended currently, and the lowest concentration of chlorine that reliably inactivates S aureus, S choleraesuis, and P aeruginosa is 100 ppm.
Research Data

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Keywords:
Chlorine chemistry; Drug Stability; Drug Storage; Microbial Sensitivity Tests; Sodium Hypochlorite chemistry; Solutions; Chlorine; Disinfection; Sodium Hypochlorite
Repository Posting Date:
28-Oct-2011
Date of Publication:
17-Oct-2011

Full metadata record

DC FieldValue Language
dc.typeResearch Studyen_GB
dc.titleStability and bactericidal activity of chlorine solutionsen_GB
dc.identifier.urihttp://hdl.handle.net/10755/175488-
dc.description.abstract<table><tr><td colspan="2" class="item-title">Stability and bactericidal activity of chlorine solutions</td></tr><tr><td colspan="2" class="item-abstract">OBJECTIVES: To determine the stability of sodium hypochlorite (diluted household bleach) when stored for 30 days in various types of containers and to determine the efficacy of low concentrations of free available chlorine to inactivate test bacteria. DESIGN: Laboratory-based study. Solutions of standard household bleach were prepared using tap water or sterile distilled water at dilutions of 1:100, 1:50, and 1:5. Chlorine concentrations were measured, and then the solutions were placed into five polyethylene containers and left at room temperature (20 degrees C) under various conditions (translucent containers with light exposure and with or without air; brown opaque container without light or air exposure). Samples for chlorine and pH determinations were taken at time 0 and on days 7, 14, 21, 30, and 40. Bactericidal activity of chlorine solutions was assessed using the Association of Official Analytical Chemists Use-Dilution Method. Test bacteria included Pseudomonas aeruginosa, Staphylococcus aureus, and Salmonella choleraesuis. RESULTS: Chlorine concentrations at 30 days varied from the 40% to 50% range for 1:50 or 1:100 dilutions stored in containers other than closed brown containers to 83% to 85% for the 1:5 dilution stored in closed but non-opaque containers to 97% to 100% for 1:50 or 1:5 solutions stored in closed brown containers. The lowest concentration of sodium hypochlorite solution that reliably inactivated all the test organisms was 100 ppm. CONCLUSIONS: These data suggest that chlorine solutions do not need to be prepared fresh daily, as is recommended currently, and the lowest concentration of chlorine that reliably inactivates S aureus, S choleraesuis, and P aeruginosa is 100 ppm. </td></tr><tr><td colspan="2" class="researcher-header">Research Data</td></tr><tr><td colspan="2" class="researcher-data"><hr/></td></tr><tr class="data"><td class="label">Ending Year:</td><td class="value"></td></tr><tr class="data"><td class="label">Design:</td><td class="value"></td></tr><tr class="data"><td class="label">Study Type:</td><td class="value"></td></tr><tr class="data"><td class="label">Theoretical Framework:</td><td class="value"></td></tr><tr class="data"><td class="label">Description of Sample:</td><td class="value"></td></tr><tr class="data"><td class="label">Sample Size:</td><td class="value"></td></tr><tr class="data"><td class="label">Number of Groups:</td><td class="value"></td></tr><tr class="data"><td class="label">Sampling Plan:</td><td class="value"></td></tr><tr class="data"><td class="label">Gender:</td><td class="value"></td></tr><tr class="data"><td class="label">Minimum Age:</td><td class="value"></td></tr><tr class="data"><td class="label">Maximum Age:</td><td class="value"></td></tr><tr class="data"><td class="label">Data Collection Settings(s):</td><td class="value"></td></tr><tr><td colspan="2" class="researcher-data"><hr/></td></tr></table>en_GB
dc.subjectChlorine chemistryen_GB
dc.subjectDrug Stabilityen_GB
dc.subjectDrug Storageen_GB
dc.subjectMicrobial Sensitivity Testsen_GB
dc.subjectSodium Hypochlorite chemistryen_GB
dc.subjectSolutionsen_GB
dc.subjectChlorineen_GB
dc.subjectDisinfectionen_GB
dc.subjectSodium Hypochloriteen_GB
dc.date.available2011-10-28T00:22:31Z-
dc.date.issued2011-10-17en_GB
dc.date.accessioned2011-10-28T00:22:31Z-
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