DNA Methylation and Immune Profiling in Active Duty/Veteran Women with PTSD

2.50
Hdl Handle:
http://hdl.handle.net/10755/308340
Category:
Abstract
Type:
Presentation
Title:
DNA Methylation and Immune Profiling in Active Duty/Veteran Women with PTSD
Author(s):
Ormeño, Erica Jane
Lead Author STTI Affiliation:
N/A
Author Details:
Erica Jane Ormeño, RN, Collin@livemail.uthscsa.edu
Abstract:

Poster presented on: Sunday, November 17, 2013, Saturday, November 16, 2013

The purpose of this pilot study conducts high-throughput epigenetic, immunologic studies that will test hypotheses directly relevant to the improved care of female VA patients and advance novel insights regarding the determinants of PTSD response and immune dysregulation. It will set the stage for intervention design based on identification of risk markers in vulnerable female soldiers and veterans.

Subjects include female veterans categorized into four study groups: control, PTSD, PTSD + adverse childhood events-sexual abuse, and only adverse childhood events-sexual abuse. Total sample size= 20. Exclusion criteria for both studies include; contraception use, pregnancy, current smoker status, substance abuse, and the anti-inflammatory/ autoimmune disease medication use. Methods: (a) Psychometric analyses: Instruments to be administered include Symptom Checklist-90-Revised, BDI-II, PCL-C, and the CTQ. (a) Methylation analyses: DNA  isolated from serum and whole genome methylation analyses will be conducted using a Illumina 450K methylation beadchip, using manufacturers protocols. (b) Biomarker/immunological analyses: A Luminex multiplexed high-sensitivity assay to determine levels of several cytokines including IL6, IFNa, IL1b, IL2, IL-4, IL-10 and TNFa. Additionally, we will assess cortisol, DHEAS in plasma, white blood cell count, and ESR.

As there are almost two million women veterans in the United States, polytrauma is of significance relevance to health care delivery in both the VA and health care agencies throughout the country. Prior trauma “primes” the inflammatory response system so that it reacts more rapidly to subsequent life stressors. Women in the military and female veterans may have experienced multiple traumatic experiences, many occurring in early childhood then later compounded by experiences in war zone exposure in a prolonged counter-insurgency environment. Few studies involving gender as a risk factor for combat related PTSD have been conducted. Pinpointing the biologic mechanisms by which gender specific alterations arise could provide fundamental insights into the etiology and treatment of PTSD.

Keywords:
IMMUNOLOGIC; FEMALES; STRESS
Repository Posting Date:
19-Dec-2013
Date of Publication:
19-Dec-2013
Conference Date:
2013
Conference Name:
42nd Biennial Convention
Conference Host:
Sigma Theta Tau International, the Honor Society of Nursing
Conference Location:
Indianapolis, Indiana, USA
Description:
42nd Biennial Convention 2013 Theme: Give Back to Move Forward. Held at the JW Marriott
Note:
This is an abstract-only submission. If the author has submitted a full-text item based on this abstract, you may find it by browsing the Virginia Henderson Global Nursing e-Repository by author. If author contact information is available in this abstract, please feel free to contact him or her with your queries regarding this submission.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen_GB
dc.type.categoryAbstracten_GB
dc.typePresentationen_GB
dc.titleDNA Methylation and Immune Profiling in Active Duty/Veteran Women with PTSDen_GB
dc.contributor.authorOrmeño, Erica Janeen_GB
dc.contributor.departmentN/Aen_GB
dc.author.detailsErica Jane Ormeño, RN, Collin@livemail.uthscsa.eduen_GB
dc.identifier.urihttp://hdl.handle.net/10755/308340-
dc.description.abstract<p>Poster presented on: Sunday, November 17, 2013, Saturday, November 16, 2013</p>The purpose of this pilot study conducts high-throughput epigenetic, immunologic studies that will test hypotheses directly relevant to the improved care of female VA patients and advance novel insights regarding the determinants of PTSD response and immune dysregulation. It will set the stage for intervention design based on identification of risk markers in vulnerable female soldiers and veterans. <p>Subjects include female veterans categorized into four study groups: control, PTSD, PTSD + adverse childhood events-sexual abuse, and only adverse childhood events-sexual abuse. Total sample size= 20. Exclusion criteria for both studies include; contraception use, pregnancy, current smoker status, substance abuse, and the anti-inflammatory/ autoimmune disease medication use. Methods: (a) Psychometric analyses: Instruments to be administered include Symptom Checklist-90-Revised, BDI-II, PCL-C, and the CTQ. (a) Methylation analyses: DNA  isolated from serum and whole genome methylation analyses will be conducted using a Illumina 450K methylation beadchip, using manufacturers protocols. (b) Biomarker/immunological analyses: A Luminex multiplexed high-sensitivity assay to determine levels of several cytokines including IL6, IFNa, IL1b, IL2, IL-4, IL-10 and TNFa. Additionally, we will assess cortisol, DHEAS in plasma, white blood cell count, and ESR. <p>As there are almost two million women veterans in the United States, polytrauma is of significance relevance to health care delivery in both the VA and health care agencies throughout the country. Prior trauma “primes” the inflammatory response system so that it reacts more rapidly to subsequent life stressors. Women in the military and female veterans may have experienced multiple traumatic experiences, many occurring in early childhood then later compounded by experiences in war zone exposure in a prolonged counter-insurgency environment. Few studies involving gender as a risk factor for combat related PTSD have been conducted. Pinpointing the biologic mechanisms by which gender specific alterations arise could provide fundamental insights into the etiology and treatment of PTSD.en_GB
dc.subjectIMMUNOLOGICen_GB
dc.subjectFEMALESen_GB
dc.subjectSTRESSen_GB
dc.date.available2013-12-19T17:30:03Z-
dc.date.issued2013-12-19-
dc.date.accessioned2013-12-19T17:30:03Z-
dc.conference.date2013en_GB
dc.conference.name42nd Biennial Conventionen_GB
dc.conference.hostSigma Theta Tau International, the Honor Society of Nursingen_GB
dc.conference.locationIndianapolis, Indiana, USAen_GB
dc.description42nd Biennial Convention 2013 Theme: Give Back to Move Forward. Held at the JW Marriotten_GB
dc.description.noteThis is an abstract-only submission. If the author has submitted a full-text item based on this abstract, you may find it by browsing the Virginia Henderson Global Nursing e-Repository by author. If author contact information is available in this abstract, please feel free to contact him or her with your queries regarding this submission.en_GB
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